Development and Evaluation of a Real-Time TaqMan-PCR for the Detection of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients

lranian journal of Clinical Infectious Diseases

B Chahardouli¹  H Ghaffari¹  M Dehghan¹  A Gharebaghian²  K Alimoghaddam¹  A Ghavamzadeh¹  O Obeidi²  AR Shamshiri¹ 

[1] Assistant Professor, , Hematology, Oncology and Bone Marrow Transplantation Research Center; Tehran University of Medical Sciences, Tehran, Iran;Assistant Professor, , Hematology, Oncology and Bone Marrow Transplantation Research Center; Tehran University of Medical Sciences, Tehran, Iran Research Assistant, Iran Blood Transfusion Organization Research Center, Tehran, Iran

关键词: Cytomegalovirus;    TaqMan PCR;    pp65 Antigenemia;    Bone Marrow Transplantation;   

DOI  :  10.17795/semj20407

学科分类：基础医学

来源: Shiraz University of Medical Sciences

【 摘 要 】

Introduction:Cytomegalovirus(CMV)hasbeenrecognizedasthemostimportantviralpathogeninpersonsundergoingbonemarrowtransplantation(BMT).Inthisstudy,wepresentthedevelopmentofaTaqMan-basedreal-timePCRassaytoquantifyhumancytomegalovirus(CMV)DNAinperipheralbloodleukocytes(PBLs)ofbonemarrowtransplantationpatients.MaterialsandMethods:Aplasmidcontainingthetargetsequencefromthepp65region(UL83)ofCMVwasconstructedasapositivecontroltemplate.Serialdilutionsof107to101plasmidsperassaywereprepared.Peripheralbloodsampleswerecollectedfrompatientsaftertransplantation.CMVDNAwasquantifiedbyRQ-PCRinparallelwiththepp65antigenemiaassayinPBLsamples.Results:Thereal-timePCRassaycoulddetectCMVDNAinpatient'ssampleswithawidelinearrange,from10toover107copiesofCMV.Real-timePCRassayresultscorrelatedwiththoseoftheCMVpp65antigenemiaassay(P<0.0001).Discussion:TheTaqManassaymaybeausefultoolforrapidquantificationofCMVinfectionandformonitoringofCMVreactivationinbonemarrowtransplantationrecipients.Theresultsofbothquantitativeassaysweresignificantlycorrelated;however,theRQ-PCRassaywasmoresensitivethanthepp65antigenemiaassay.

【 授权许可】

Unknown