期刊论文详细信息
lranian journal of Clinical Infectious Diseases | |
A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human | |
MH Roustai1  H Soleimanjahi1  M Ziyaeyan1  F Sabahi1  A Mostafaee2  M Ravanshad1  | |
[1] Department of Medical Virology, Faculty of Medicine, Tarbiat Modarres University, Tehran, Iran.;Department of Medical Virology, Faculty of Medicine, Tarbiat Modarres University, Tehran, Iran. Department of Immunology, Faculty of Medicine, Kermanshah University, Kermanshah, Iran. | |
关键词: Herpes Simplex Virus; ELISA; IgM; | |
DOI : 10.17795/semj20366 | |
学科分类:基础医学 | |
来源: Shiraz University of Medical Sciences | |
【 摘 要 】
Anantibody-captureenzyme-linkedimmunosorbentassay(ELISA)basedonthereactionbetweenantihumanIgMandserumIgMandHSV-1conjugatedwithhorseradishperoxidase(HRP)wasdeveloped.ThespecificityandsensitivityofthedevelopedHSV-1IgMELISAwereevaluatedandcomparedwithacommerciallyavailabletestkitbyusingsetsofwelldefinedseraofknownHSV-1primaryinfection.Forvalidation,526serumsampleshavebeentested.TheHSV-1IgMELISAhadacceptablesensitivityandspecificity,whichwerecomparablewiththatofthecommercialtestkit.Bothassaysshowedsomecross-reactivitywithseracontaininganti-HSV-2antibodies
【 授权许可】
Unknown
【 预 览 】
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RO201912090831862ZK.pdf | 53KB | download |