In vitro Variability of Morphogenetic Responses of Tomato (Lycopersicon esculentum Mill.) Explants under Salt Stress and Thidiazuron as Plant Growth Regulator

Journal of Applied Biological Sciences

Amar TOUATI¹  Fatima Zohra FYAD-LAMECHE¹  Sara LACHACHI¹  Nourredine YAHIA¹ 

关键词: Salt stress;    Cytokinins;    Organogenesis;    Somatic Embryogenesis.;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Forthepresentinvestigation,sixgenotypesoftomato(LycopersiconesculentumMill.)widelygrowninAlgeriawereselectedandusedasresearchmaterialtostudythemorphogeneticvariabilityunderstressconditionsusingdifferentmediatreatmentsandtwotypesofexplants(cotyledonsandhypocotyls)inordertoevaluatetheirresponsestocallusinduction,embryosproductionandcaullogeniccapacity.Invitrogrowthseedlingsagedfor21daysoldwereusedassourceofexplants.TheseexplantswerecultivatedintoaMS-basedmediumcontainingaconstantconcentrationofTDZ(3mg/L)usedasCytokininsPGR,combinedornotwithfourincreasinglevelsofNaClat0,25,50and75mMforeightweeks.ThisCytokininspredisposestheexplanttostress.Statisticalanalysisindicatedahighlysignificantvariationsinresponsesaccordingthenatureofgenotype,treatmentmedium,explantoriginandinteractionsthereofpossessampleinfluenceonmorphogeneticcharactersundertheseconditions.ResultsshowedthatthemorphogenesisresponsesseemstobedependentNaCllevelsaddedinthemedia,amoderateconcentrationsincluding25and50mMinducescomparedtocontrolmedium.Whereas,mediumMT3hadshownarelativetopoorresponseforthemostgenotypestested.ItisinterestingthatthemoderatecombinationNaCl/TDZwasbetterfortheembryosproduction,whichcanbeuseddirectlyorconvertedintoartificialseedsforpropagationofhighvaluehybrid.Allmosteverytestedexplantweredifferentiatedintocallusbutthefavoriteexplantforcarriedaresearchonthevariabilityunderstressconditionsandembryogeniccapacityisthecotyledon.Differencesamonggenotypesweredetected.Foralleffectsanalyzed,homogeneousgroupswereestablishedusingNewman-Keulspost-hottest.ThisattemptmaybeusedasefficientprotocolforgeneticimprovementandtheuseofcombinationPGRs/Stressoragentcouldbetheoptionforthesomaticembryogenesisinvestigations.

【 授权许可】

Unknown