Microbial Potential of Lipase production from Different Industrial Effluents

Journal of Applied Biological Sciences

Azra YASMIN¹  Shaheen SHAHZAD¹  Ifrah SHAFQAT¹ 

关键词: Lipase;    Industrial effluents;    Acinetobacter junni;    Acinetobacter lwoffi;    Alishewanella agri;    SDS-PAGE;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Lipasesholdsignificantpositioninthefieldofbiotechnologyduetoitsabilitytocatalyzeesterification,interesterification,andtransesterificationreactioninnonaqueousmedia.Inthepresentstudyfourdifferentlipaseproducingbacteriawereisolatedfromeffluentsofleather,marbleandtextileindustry.Allthestrains(L9,L15,L16andL20)isolatedweregramnegativeandsubjectedtobothbiochemicalandmolecularcharacterization.BLASTresultsfor16SrDNAsequencesrevealedthatL9(KJ748674)strainhad99%similaritywithAcinetobacterlwoffi.L15(KJ748675)andL16(KJ864927)strainsshowed98%and99%sequencehomologywithAcinetobacterjunniandstrainL20(KJ873872)belongstoAlishewanellaagrisp.Forenzymeproductionstudies,pHandtemperaturewereoptimizedontwodifferentenrichmentmedia.Comparisonoftwodifferentenrichmentmediashowedthatmediumcontainingpeptone,yeastextract,NaClandoliveoilgaveoptimumproductionforallthestrains.AlishewanellaagriL20(isolatedfromtextileindustryeffluent)hadgrowthatalltemperatures(30,37,42and45°C)inmediumcontainingyeastextract,NaClandoliveoil.Incomparisontoallthereportedstrainsofthepresentstudy,Alishewanellaagri(L20)hadmaximumenzymeproductionof17U/mlatelevatedtemperatureof45°C.PartialpurificationandSDS-PAGEanalysisshowedthatproteinbandsoflipaseswererangedbetween35-66.2kDa.Itisevidentthathighcostoflipasehasgenerallyreduceditsexploitationatindustriallevel.Therefore,screeningandidentificationoftheselipolyticstrainswillactasasignificantadditiontothedatabaseonlipaseresearchanditsapplicationatindustriallevel.

【 授权许可】

Unknown