Effects of Sodium Benzoate and Citric Acid on Serum, Liver and Kidney Tissue Total Sialic Acid Levels: An Ultrastructural Study

Journal of Applied Biological Sciences

Tülin AKTAǹ  Elvan BAKAR¹ 

关键词: sialic acid;    citric acid;    sodium benzoate;    liver;    kidney;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Inthisstudy,itwasaimedtoinvestigatethemorphologiceffectsofsodiumbenzoate(SB)andcitricacid(CA),additivesusedinfood,onratliverandlivertissuesandchangesintotalsialicacid(TSA)levels.Atotalof30Wistaralbinoratsweighing200-250grweredividedto3groupswith10ineach.Ratsincontrolgroup,SBgroupandCAgroupwereadministeredonbygavagetapwater,Na-benzoate(2442mg/kgbodyweight)andcitricacid(576mg/kgbodyweight)oncedailyrespectivelyfor10days.Attheendoftheexperiment,animalsincontrolandexperimentgroupswereopenedandheartblood,hepaticandrenaltissueswereobtained.Removedtissueswereexaminedunderlightandelectronmicroscopyfollowingroutinefollowupprocedures.TSAamountsinserumandtissuesweremeasuredwithspectrophotometricmethod.InSBandCAgroups,disruptionwasobservedinhepatictissueengineering,vacuolizationwasobservedinhepatocytesandalsopyknoticnuclei,nucleuslossesandhypertrophywereobserved.InSBandCAgroups,pyknoticnucleiwereobservedinrenaltubularcells,membraneinjurywasobservedinapicalandbasalsurfacesoftubularcells,degenerationwasobservedinglomerularstructuresandirregularitywasseenintubularstructures.Plasmarhexis,lossesinmitochondrialcrista,degenerationinoutermembraneandnuclearmembraneofmitochondria,vacuolizationinhepatocytesandmyelinfigureswereobservedelectromicroscopicallyduetoSBandCA.Inrenaltissues,electronmicroscopicdegenerativechangesincludedelectron-densegranules,cristalossesinmitochondria,irregularmicrovilli,vacuolizationandplasmarhexisincytoplasmaoftubularcells,irregularityinchromatinmaterial,disruptioninbasalmembrane,enlargementinERsacs.InSBgroups,serumTSAamountwasseentodecreasesignificantly(p<0.05)andasignificantdifferencewasnotseeninCAgroup.ItwasseenthatsialicacidamountssignificantlydecreasedbothinliverandkidneytissuesinSBgroup(p<0.0001);inCAgroup,whileasignificantdifferencewasnotseeninhepatictissues,renalTSAlevelwasdetectedtoincreasesignificantly(p<0.0001).WheneffectsofSBandCAonTSAamountandmorphologicdegenerationsinliverandrenalkidneywereevaluatedtogether,weconsiderthatmoredetailedstudiesarerequiredabouttheuseofthesefoodpreservatives.

【 授权许可】

Unknown