The Effects of Nonylphenol on Gamete Physiology in Bovine

Journal of Applied Biological Sciences

Cevdet UĞUZ¹  Hakan SAĞIRKAYA¹  Selim ALÇAY¹  Burcu ÜSTÜNER¹  Selcen Süheyla ERGÜN¹ 

关键词: Nonylphenol;    sperm;    oocyte;    Tunel Assay;    endocrine distruptors;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Alkylphenolethoxylates(APEOs)areusedasnon-ionicsurfactantsinvarietyofindustrial,agricultiralanddomesticproductssuchaspesticides,detergents,paintsandcosmetics.Therefore,thesecompoundscanreachtohumanbeingthroughfoodchains.TheseendocrinedisurptersalsocalledXenoestogenhaveestrogenic,carsinogenicandtoxiceffects.Nonylphenol(NP)exertsitsestrogeniceffectsbybindingtoestrogenreceptors.NPcausesmorphologicalandfunctionalalterationsinmaleandfemalegenitaltractandmammaryglands.Thesealterationsmayinduceinfertility,mammaryandprostatecancer.Therefore,themainpurposeofthisstudyistodeterminetheadverseeffectsofNPonspermandoocytes.TheeffectsenvironmentallyrelevantNPconcentrationssuchas0.01,0.1,1,10and100μgNP/mlwereemployed.NPmediatedabnormalitiesinspermDNAandoocytematurationwereinviestigated.TunelassaywasperformedtodeterminetheadverseeffectsofNPonspermDNA,whereastheeffectsofNPonoocytematurationinTCM-199weretested.Thepresentstudydemonstratedthat100μgNP/mlconcentrationinducedapoptosisbycausingDNAbreaksinbovinespermcells.Thisstudyalsoshowedthat100μgNP/mlconcentrationsinhibitsoocytematuration.ItisconcludedthatNPhaveadverseeffectsontheintegrityofspermDNAandoocytematuration.

【 授权许可】

Unknown