In vitro Plant Regeneration Influence by BAP and IBA in Lentils (Lens culinaris Medik)

Journal of Applied Biological Sciences

Musa TÜRKER¹  Fethi Ahmet ÖZDEMİR¹ 

关键词: Acclimatisation;    auxins;    cytokinins;    Lentil;    micropropagation;    rooting;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Lentilisanimportantfoodlegumecropthatisveryrecalcitranttoshootregenerationandrootingunderinvitroconditions.Thisstudyaimedtodevelopefficientandreliableprotocolforinvitroplantregeneration.ThereulstsofthisstudyshowedthatInthiscontext,16Turkishlentilcultivarswereshoottip,stem,hypocotyl,cotyledonandrootasexplants.TheMSmediumcontaing4mg/lBAPinducedmaximumnumber(8.25)ofshootspershoottipexplantofcv.Yesil21.Whereaas,maximumnumber(28.33)ofshootspercotyledonexplantofcv.OzbekwasinducedonMSmediumcontaining1mg/lIBA.However,IBAderivedshootswereeasytorootonMSmediumcontaining1.87mg/lNAA.Theregeneratedexplantsweretransferredtogreenhouseforacclimatisation,floweringandseedset.Itwasconcludedthatinvitroshootregenerationoflentils(LensculinarisMedikus)wasstronglyinfluencedbythetypeofexplant,plantgrowthregulatorconcentrationandcombinations.

【 授权许可】

Unknown