期刊论文详细信息
Journal of Applied Biological Sciences
Efficient in vitro Microprapagation for the Conservation of Endemic and Endangered aucher-eloy grape hyacinth [Muscari Aucheri (boiss.) Baker]
Serkan URANBEY1  Cengiz SANCAK1  Parizad Allahverdikhan VAZİRİ1 
关键词: Bulblets;    bulb scales;    in vitro;    micropropagation;    Muscari aucheri;   
DOI  :  
学科分类:生物科学(综合)
来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente
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【 摘 要 】

Muscariaucheri(Boiss.)BakerfamilyLiliaceaeisendemictoTurkeyandisthreatenedbycompleteextinction.Toconservetheplant,inaccordancewithinternationalagreementsfortheprotectionofendangeredgeophytes,collectionofM.aucheribulbsfromthenaturalhabitatsisforbiddeninTurkey.Duetopoordiversitythenaturalpropagationrateoftheplantisrelativelyslowandittakes4-5yearstodevelopabulbofcommercialsize;whichrestrictslarge-scalecultivationoftheplant.Developmentofconservationmethodslikeinvitropropagationtechniques,forconservationhavelowimpactonwildpopulationsastheyuseaminimumofplantmaterial.Thepresentstudyaimedtoestablishastrategyforefficientinvitrobulbletregenerationofthisimportantgeophyteusing4scalebulbexplantsasanaidtobreedingandmultiplicationoftheplants.TheseexplantswereculturedonMSmediumcontaining0.5,1.0,2.0mg/lBAP,0.5,1.0,2.0mg/lGA4and0.1,0.2mg/lIAA.ThemaximumnumberofbulbletsperexplantwasinducedonMSmediumcontaining0.5mg/lBAP,0.2mg/lIAA.ThebulbletsregeneratedonanyvariantofBAP-IAAandGA4-IAAweresuccessfulyrootedonMSmedium.About17%oftherootedbulbletscouldbeacclimatised.Allacclimatizedplantssurvivedandshowedflourishinggrowthunderfieldconditions.Thusthisprotocolcouldbeusedineffectivecanmultiplicationandconservationoftheplantunderinvitroconditions.

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