Efficient in vitro Microprapagation for the Conservation of Endemic and Endangered aucher-eloy grape hyacinth [Muscari Aucheri (boiss.) Baker]

Journal of Applied Biological Sciences

Serkan URANBEY¹  Cengiz SANCAK¹  Parizad Allahverdikhan VAZİRݹ 

关键词: Bulblets;    bulb scales;    in vitro;    micropropagation;    Muscari aucheri;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Muscariaucheri(Boiss.)BakerfamilyLiliaceaeisendemictoTurkeyandisthreatenedbycompleteextinction.Toconservetheplant,inaccordancewithinternationalagreementsfortheprotectionofendangeredgeophytes,collectionofM.aucheribulbsfromthenaturalhabitatsisforbiddeninTurkey.Duetopoordiversitythenaturalpropagationrateoftheplantisrelativelyslowandittakes4-5yearstodevelopabulbofcommercialsize;whichrestrictslarge-scalecultivationoftheplant.Developmentofconservationmethodslikeinvitropropagationtechniques,forconservationhavelowimpactonwildpopulationsastheyuseaminimumofplantmaterial.Thepresentstudyaimedtoestablishastrategyforefficientinvitrobulbletregenerationofthisimportantgeophyteusing4scalebulbexplantsasanaidtobreedingandmultiplicationoftheplants.TheseexplantswereculturedonMSmediumcontaining0.5,1.0,2.0mg/lBAP,0.5,1.0,2.0mg/lGA4and0.1,0.2mg/lIAA.ThemaximumnumberofbulbletsperexplantwasinducedonMSmediumcontaining0.5mg/lBAP,0.2mg/lIAA.ThebulbletsregeneratedonanyvariantofBAP-IAAandGA4-IAAweresuccessfulyrootedonMSmedium.About17%oftherootedbulbletscouldbeacclimatised.Allacclimatizedplantssurvivedandshowedflourishinggrowthunderfieldconditions.Thusthisprotocolcouldbeusedineffectivecanmultiplicationandconservationoftheplantunderinvitroconditions.

【 授权许可】

Unknown