Optimization of Sterilization and Micropropagation of Water Lettuce (Pistia stratiotes L.)

Journal of Applied Biological Sciences

Khalid Mahmood KHAWAR¹  Muhammet DOGAN¹  Mehmet KARATAS¹  Muhammad AASIM¹ 

关键词: Ex vitro;    plant growth regulators;    micropropagation;    sterilization;    water lettuce;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Waterlettuce(Pistiastratiotes)isanimportantmedicinalaquaticplantthatisalsousedforphytoremediationorasbioindicator.Itisverydifficulttomultiplytheplantusingmicropropagationtechniquesduetohighcontaminations.Thestudyaimedtoalleviatethisproblemforefficientmultiplicationoftheplantsubsequently.Thestudymadeuseof60%commercialbleach(5%NaOCl)or60%H2O2forsurfacesterilizationfor10minfollowedbyadding500mg/lDuocid®(awidespectrumantibiotic)intheculturemedium.Theresultsshowedthatcommercialbleachwasnotpropersterliserasallexplantsbleached;whereas,60%H2O2basedsurfacesterilizationwasmostoptimumforthepurpose.TheSurfacesterilizedplantswereinvitroculturedonagarsolidifiedorliquidMSmediumcontaining0.05-0.40mg/lkinetin.Centpercentshootregenerationfrequencywasonbothagarsolidifiedorliquidmedium.However,allexplantsonagarsolidifiedorliquidmediumhadtenancytodieturningallofthemtoalbino.Therefore,multiplicationunderexvitroconditionsonliquidMSmediumcontaining0.05-0.40mg/lkinetinorBAPusingunsterilizedshootmeristemswithoutaddingsucrose.Subsequently,theexplantsinduced2.0-40plantletsperexplanton2.0-3.0mg/lkinetinorBAPcontainingculturemedium.Theplantsregeneratedonbothculturemediawerecomparablewithplantsgrowingundernaturalconditionsanddidnotshowanysignofstresscausingalbinismornecrosis.

【 授权许可】

Unknown