Examination of the Genotoxic Effects of Various Parabens Used as Food Additives with the Drosophila Wing Spot Test (SMART)

Journal of Applied Biological Sciences

Handan UYSAL¹  Arif AYAR¹ 

关键词: Drosophila melanogaster;    ethylparaben;    butylparaben;    genotoxicity;    SMART;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Theobjectiveofthisstudyistodeterminethepossiblegenotoxiceffectsofthepara-hydroxybenzoicacidesters(Parabens)ofethylparabenandbutylparabenusedaspreservativesubstancesinthefood,cosmeticanddrugindustriesonDrosophilamelanogasterwiththeSomaticMutationandRecombinationTest(SMART).Inourstudy,twodifferentmutantstrainsofD.melanogasterknownasthefruitflywithrecessiveflr3andmwhidentifiergenesinitsgenomewereused.Thetrans-heterozygouslarvaeof72±4hoursobtainedasaresultofthecrossbreedingbetweenthesetwomutantstrainswerefedchronicallywithvariousconcentrations(100,150,200and250mM)ofethylparabenandbutylparaben.Inaddition,experimentalsetupsforcontrolgroupshavebeenpreparedusingethylmethanesulfonate(EMS)anddistilledwater.Wingpreparatesofthefull-grownindividualsdevelopedfromtheselarvaehavebeenpreparedandthesepreparateshavebeenexaminedunderamicroscope.Accordingtothedataobtainedfrommicroscopicanalysis,nosignificantincreasehasbeendeterminedinalltypeclonenumberofethylparabenandbutylparabengroupsincomparisonwiththecontrolgroup.Thishasbeendeterminedtohavenegative(-)orinsignificant(i)effectsonthecontrolgroupstatistically(P>0.05).Asaresultofthedataweobtained,ithasbeenconcludedthatparabensshowtoxiceffects,althoughnogenotoxiceffectsonD.melanogaster.

【 授权许可】

Unknown