RFL polymorphism of three fertility genes in Egyptian buffalo

Journal of Applied Biological Sciences

Othman E. OTHMAN¹  Mohamed F. ABDEL-SAMAD¹ 

关键词: Buffalo;    FSHR;    LHR;    ERα;    PCR-RFLP;    Nucleotide sequences;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

ThereisaconsiderableinterestintheapplicationofmoleculargeneticstechnologiesintheformofspecificDNAmarkersthatareassociatedwithvariousproductivitytraitstopromotemoreefficientandrelativelyeasyselectionandbreedingoffarmanimalswithanadvantageforinheritabletraitsofproductionandreproduction.Riverbuffaloisaspeciesofgreateconomicpotential,especiallyindevelopingcountries;itisthemainsourceofmeatandmilkinEgypt.Thisstudyaimedtodetectthegeneticpolymorphismofthreegenes;follicle-stimulatinghormonereceptor(FSHR),luteinizinghormonereceptor(LHR)andestrogenreceptor-α(ERα)geneswhichareconsideredcandidategenesforfertilityandreproductionperformances.ThegeneticpolymorphismdetectionofthesegenesinEgyptianbuffaloeswillenableustoidentifyandsubsequentlyselecttheanimalswithhighfertilityperformances-dependingonthesemolecularmarkers-forbreedingprograms.GenomicDNAextractedfrom100healthyfemalebuffaloeswasamplifiedusingprimersthatweredesignedfromthecattleFSHR,LHRandERαgenesequences.TheamplifiedfragmentsofFSHR(306-bp),LHR(303-bp)andERα(248-bp)weredigestedwithAluI,HhaIandBglIrestrictionenzymes,respectively.TheresultsshowedthatalltestedbuffaloesaregenotypedasCCforFSHRandTTforLHR.RegardingtotheERαgene,18%oftheinvestigatedbuffaloesaregenotypesasheterozygousAGandtheremaininganimals(82%)aregenotypedasGG.Tothebestofourknowledge,thisisthefirstpublisheddataonthegeneticcharacterizationandnucleotidesequencesoffertilitygenesinEgyptianbuffalo.ThenucleotidesequencesofEgyptianbuffaloFSHRC,LHRT,ERαGandERαAallelesweresubmittedtonucleotidesequencesdatabaseNCBI/Bankit/GenBankandhavetheaccessionnumbersJX049145,JQ885687,JQ308795andJQ308796,respectively.

【 授权许可】

Unknown