Genetic Polymorphism of Whey Protein Genes β-LG and α-LA in Three Egyptian Sheep Breeds

Journal of Applied Biological Sciences

Othman E. OTHMAN¹  Samia A. El-FIKY¹  Eman R. MAHFOUZ¹  Esraa A. BALABEL¹  Nagwa A. HASSAN¹ 

关键词: Sheep;    β-LG;    α-LA;    RFLP;    SSCP;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Themajorwheyproteins,β-lactoglobulin(β-LG)andα-lactalbumin(α-LA),arestronglycorrelatedwiththenutritionalvalueandthefunctionalpropertiesofmilkwhey.Ovinewheyhasauniqueproteincompositioncomparedtothatofbovinewheyanditisespeciallyrichinproteins.Geneticpolymorphismofβ-LGusingPCR-RFLPandα-LAusingPCR-SSCPwasanalyzedinthreenativeEgyptiansheepbreedsnamedBarki,RahmaniandOssimi.Digestionofresultingamplifiedfragmentsofβ-LG(452-bp)withRsaIendonucleasedifferentiatedbetweenthreedifferentgenotypesAA(69.77%),AB(26.74%)andBB(3.49%).TheresultsshowedthatAallelewaspresentintheEgyptiansheepbreedswithafrequency(83.14%)higherthanBallele(16.86%).ABallelesappearedatfrequencies70.4%29.6%,95.0%5.0%and82.8%17.2%inBarki,RahmaniandOssimi,respectivelySSCPresultsoftheα-LAamplifiedfragments(166-bp)showedthatthethreenativeEgyptiansheepbreedspossesstwodifferentpatternsrelatedtothedifferenceinnucleotidesequenceswithfrequencies88.1%and11.9%.Thefrequenciesofpattern1rangedfrom81.48%inBarkito89.66%inOssimiand92.86%inRahmanibreedswhereasthepattern2waspresentatfrequencies18.52%,7.14%and10.34%inBarki,RahmaniandOssimibreeds,respectively

【 授权许可】

Unknown