Multi-Spectroscopic Investigation of the Interactions between Cholesterol and Human Serum Albumin

Journal of Applied Biological Sciences

M. M. ABU-HADID¹  J. GHITHAN¹  S. DARWISH¹  M. M. Abu TEIR¹ 

关键词: cholesterol;    amide I;    amide II;    amid III ;    binding mode;    binding constant;    protein secondary structure;    Fourier transform IR;    UV–;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Theinteractionbetweencholesterolandhumanserumalbuminhasbeeninvestigated.ThebasicbindinginteractionwasstudiedbyUVabsorptionandfluorescencespectroscopy.FromspectralanalysischolesterolshowedastrongabilitytoquenchtheintrinsicfluorescenceofHSAthroughastaticquenchingmechanism.Thebindingconstant(k)isestimatedtobeK=0.214×104M-1at293K.FTIRspectroscopywithFourierself-deconvolutiontechniquewasusedtodeterminetheproteinsecondarystructureandcholesterolbindingmechanisms.TheobservedspectralchangesindicateahigherpercentageofH-bondingbetweencholesterolanda-helix(secondarystructuremotifinHSA)comparedtothepercentageofH-bondingbetweencholesterolandb-sheets(secondarystructuremotifinHSA).

【 授权许可】

Unknown