Evaluation of DNA Polymorphism in Egyptian Buffalo Growth Hormone and Its Receptor Genes

Journal of Applied Biological Sciences

Othman E. OTHMAN¹  Karima M. SEWIFY¹  Mohamed F. ABDEL-SAMAD¹  Nadia A. Abo EL MAATY¹ 

关键词: Buffalo;    GH;    GHR;    PCR;    RFLP;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Currently,theprimarythrustofresearchinanimalgeneticsistheidentificationofgenes(so-calledmajorgenes),whichaffecttheexpressionofquantitativetraitsmarkedly.Twoofthesemajorgenesaregrowthhormone(GH)anditsreceptor(GHR)geneswhicharerelatedtoproductionandreproductiontraitsinlivestock.ThereisextensiveliteratureonthegeneticpolymorphismofGHandGHRincattle,butperusalofliteraturehasindicatedpaucityofinformationonthesetwogenesinbuffalo.ThisstudyaimedtoevaluatethegeneticpolymorphismwithingrowthhormoneanditsreceptorgenesinEgyptianbuffalousingPCR-RFLPtechnique.GenomicDNAextractedfrom100healthybuffaloeswasamplifiedusingprimersthatweredesignedfromthecattleGHandGHRgenesequences.AllbuffaloanimalsinvestigatedinthisstudyaregenotypedasLLforGHgenewherealltestedbuffaloDNAamplifiedfragmentsat211-bpweredigestedwithAluIendonucleaseandgavetwodigestedfragmentsat159-and52-bpduetothepresenceoftheAluIrestrictionsiteatposition52^53(AG^CT).TheamplifiedfragmentsofGHRgeneobtainedfromalltestedbuffaloDNAat342-bpweredigestedwithAluIendonuclease.TheresultshowedthatalltestedbuffaloesaregenotypedasGGforGHRgene,whereamplifiedfragmentsweredigestedintotwodigestedfragmentsat241-and101-bpduetothepresenceofAluIrestrictionsiteatposition101^102(AG^CT).

【 授权许可】

Unknown