Association between Estrogen Receptor alpha Polymorphisms and Breast Cancer Risk in Jordanian Women

Journal of Applied Biological Sciences

Fawzi ALSHEYAB¹  Anas DANNOUN¹  Dalal AWAD¹  Yousef MOSAMEH¹ 

关键词: Estrogen receptor;    polymorphism;    Breast Cancer;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Estrogenreceptoralpha(ER-α)belongtothenuclearreceptorsuperfamilyofligand-inducibletranscriptionfactors.Itisinvolveinbothmammarydevelopmentandinbreastcarcinogenesis.Manypolymorphismssuggestedassociationwiththeincidenceofbreastcancer.ThemostwidelystudiedPolymorphismsarePvuIIandXbaIrestrictionsites,theylieswithinintrononeofestrogenreceptoralphagene.Thisstudywasdonetoevaluatetherelationshipoftwowell-studiedpolymorphismsintheestrogenreceptoralphagene,(PvuIIandXbaI),andtheriskofbreastcancerinJordanianwomen.Inourstudy,100bloodsamplesfromJordanianwomenwithbreastcancerhadbeenobtainedandusedforsubsequentDNAextractionandgenotypedforthePvuIIandXbaIpolymorphismsusingRestrictionFragmentLengthPolymorphism(RFLPs)technique.Also,a100bloodsamplesfromnormalJordanianwomenhadbeenusedascontrol.Forstatisticalanalysis,SPSScomputerizedprogramtocalculateP-valueforpolymorphismsandHardy-Weinbergequationforgenotypefrequencieswasused.TherewasnosignificantdifferencebetweenbreastcancerpatientsandhealthycontrolindividualsinthedistributionofthePvuIIgenotypes.TherewasnosignificantdifferencebetweenbreastcancerpatientsandhealthycontrolindividualsinthedistributionofthePvuIIgenotypes(P>0.05;P=0.27),noassociationbetweenXbaIpolymorphismandbreastcancerrisk(P>0.05;P=0.111).Alsotherewasnosignificantassociationwithhaplotypeanalysis(P>0.05;P=0.07)exceptforAGTChaplotype(P≤0.05;P=0.043)andAATChaplotype(P≤0.05;P=0.027).ThisresultsuggeststhatPvuIIandXbaIpolymorphismdoesn’tplayaroleinthesusceptibilitytobreastcancerinJordanianwomen.

【 授权许可】

Unknown