Synchronization and Determination of the Cell Cycle Phases of Breast Cancer Cell Lines Using Hydroxyurea

Journal of Applied Biological Sciences

Yavuz ÇAKIR¹  Farag GABER¹ 

关键词: Breast Cancer;    Flow Cytometry;    Cell Cycle;    Cell Synchronization;    Hydroxyurea;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Theanalysisofcellcycleincancercelllinesprovidesvaluableinformationforthediagnosisofcancer.Cellcyclesynchronizationisaneffectivewaytoanalyzedifferentcellcyclephases.Inthisstudy,bothasynchronousandsynchronousER(+)MCF-7andER(-)MDA-MB-453breastcancercellswereanalyzed.CellswerestainedusingPIandasynchronouscellcyclephaseswereanalyzedbyflowcytometer.ThenbothcelltypesweresynchronizedclosetoG1-Sphaseusing2mMHydroxyureaandsynchronouscellcyclephaseswereanalyzed.Theasynchronouscellsmovedsmoothlyandcompletedtheircyclein24h.ThesignificantinhibitionoftheDNAsynthesisinsynchronousMCF-7cellswasobservedat12haftertheremovalofHydroxyurea,andalmost28%ofthecellswereatSphasewhereasonly58%ofthecellswereinG1phase.AmarkedG1/SaccumulationalsowasobservedinsynchronousMDA-MB-453cellsimmediatelyafterremovalofHydroxyureaat0h.TheDNAsynthesiswassignificantlyblockedbecause%82ofthecellswasatG1phasewhereasonly%10ofthecellswasatSphase.DNAsynthesisresumedandcellsreturnedtonormalcellcycleoverthenext48h.Therewasnosignificant“subG1peak”indicatingapoptosisorcelldeath.ThisdataindicatesthatHydroxyureamaybeusedtosynchronizebothcellswithoutinducingapoptosisorcelldeath.

【 授权许可】

Unknown