Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch

Journal of Applied Biological Sciences

Mohammad Jahir KHAN¹  Fahim Halim KHAN¹  Qayyum HUSAIN¹ 

关键词: β amylase;    Celite-545;    immobilization;    sweet potato;    batch process;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

PresentstudydemonstratestheimmobilizationofacetonefractionatedIpomoeabatata(sweetpotato)βamylaseonaninorganicsupport,Celite-545bysimpleadsorptionmechanism.Theadsorbedenzymeexhibitedanactivityyieldof244Ug-1ofthematrixwitheffectivenessfactor‘η’0.83.InteractionbetweenCelite-545andenzymewasconfirmedbyfouriertransforminfraredspectroscopyandatomicforcemicroscopy.Thebindingefficiencyofenzymetothesupportwasanalyzedbyelutingitwith1.0MNaCl.BothsolubleandimmobilizedβamylaseexhibitedsamepHoptimawhiletemperatureoptimumofimmobilizedenzymewasshiftedfrom50oCto60oC.Theimmobilizedβamylasepreparationwassuperiortothefreeenzymeinhydrolyzingstarchinabatchprocess:ithydrolyzedstarchto88%and96%at40oCand50oC,respectivelywhereassolubleenzymehydrolyzedonly83%and80%ofstarchundersimilarexperimentalconditions.Theimmobilizedβamylaseretained84%ofitsoriginalactivityafter30daysstorageat4oC,whilethesolubleenzymeshowedonly41%oftheinitialactivityunderidenticalconditions.Immobilizedβamylaseretained79%activityevenafterits7threpeateduse.

【 授权许可】

Unknown