Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach

Journal of Applied Biological Sciences

M. M. ABU-HADID¹  J. H. GHITHAN¹  S. M. DARWISH¹  M. M. ABU TEIR¹ 

关键词: progesterone;    amide I-III ;    binding mode;    binding constant;    protein secondary structure;    Fourier transform IR;    UV–spectroscopy;    Flurosence spectroscopy.;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Theinteractionbetweenprogesteroneandhumanserumalbuminhasbeeninvestigated.ThisinteractionwasstudiedbyUV-absorptionandfluorescencespectroscopy.FromspectralanalysisprogesteroneshowedastrongabilitytoquenchtheintrinsicfluorescenceofHSAthroughastaticquenchingprocedure.Thebindingconstant(K)isestimated6.56×102M-1at293K.FT-IRspectroscopywithFourierself-deconvolutiontechniquewasusedtodetermineHSAsecondarystructureandprogesteronebindingmechanisms.TheobservedspectralchangesindicatetheformationofH-bondingbetweenprogesteroneandHSAmoleculeswhichcanberelatedtotheintensitydecreaseintheabsorptionbandofα-helixrelativetothatofβ-sheets.

【 授权许可】

Unknown