Enhanced Production and Stability of Laccase Using Some Fungi on Different Lignocellulosic Materials

Journal of Applied Biological Sciences

Murat ARDIǹ  Ahmet ÇABUK¹  Serap GEDIKLI¹  Firdevs ÖZŞÖLEN¹  Pınar AYTAR¹  Meltem ÇELIKDEMIR¹ 

关键词: Solid state fermentation;    laccase;    FTIR;    white rot fungi;    stability;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Thepotentialsofthirteenlignocellulosicwasteswheatbran,barleybran,coneofPinusnigra,sawdust,cornbran,oat,ricebran,canola,driedtea,groundcloverstraw,sunflowerstalk,soybeanbagasse,drieddistillersgrainswithsoluble(DDGS)assolidsubstratesinrespectoflaccaseproductionbythewhite-rotfungiTrametesversicolor(ATCC200801),Phanerochaetechrysospsorium(ME446)andPleurotussajor-cajuundersolid-stateconditionswereassessed.ThehighestlaccaseactivitywasdetectedwhenT.versicolorwasgrownongroundcloverstraw,showingamaximumvalueof22.36Uml-1.Thehighenzymeactivitywasobservedtocorrespondwithchemicalcompositionofsubstrateevaluatedinrespectofholocellulose,lignin,carbonandnitrogencontents.Inaddition,FTIRanalysiswasperformedtochemicalchangesofsubstratebeforeandafterusinginsolidstateculture.Furthermore,pHandthermalstabilityofenzymeobtainedfromT.versicolorATCC200801grownoncloverstrawwerestudied.Also,effectsofdifferentinhibitorsonthesameenzymeactivitywereinvestigated.

【 授权许可】

Unknown