Molecular Breeding of Selçuklu-97 Durum Wheat Cultivar for Some Genes Affecting Pasta Quality

Journal of Applied Biological Sciences

Özlem ATEŞ SÖNMEZOĞLU¹  Tuğba ESERKAYA GÜLEǹ  Ahmet YILDIRIM¹ 

关键词: Durum Wheat;    Triticum Durum;    y-gliadin 45;    LMW-2 glutenin;    Backcross Breeding;    SSR;    A-PAGE;    SDS-PAGE.;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Themostefficientwayofproducingandsupplyingproperrawmaterialdesiredbythepastaindustryisthedevelopmentofhighqualitydurumwheatvarieties.Therefore,qualityofTurkishdurumwheatvarietiesshouldbeimprovedusingmodernbreedingmethodswithoutadverselyaffectingtheiryields.Inthisstudy,importantgenes(y-gliadin45andLMW-2glutenin)affectingthequalityofpastaproductsweretransferredtoaTurkishdurumwheatvariety,Selçuklu-97,inabackcrossbreedingmethodincombinationwithmarkerassistedselection(MAS).ACanadiandurumwheatcultivarwithhighquality,Kyle,wasusedasthedonorparent.EachofF1andbackcross(BC)plantswasbackcrossedfourtimestotherecurrentparentandinallofthegenerations,backcrossedplantscarryingthetargetedQTLswereselectedbytheMAS.TheMASmethodwasemployedincombinationwithembryocultureandrapidplantgrowthinacontrolledgreenhouseconditions.Inidentifyingandtransferringprocessesofthegeneregions,molecularDNAmarkers(SSR,STSandGAG)wereemployedwithA-PAGEandSDS-PAGEmethods.A-PAGEwasusedforselectionofy-gliadin45,SDS-PAGEforselectionofLMW-2glutenins,andfourSSRprimers(Xgwm550,Xgwm608,Stm553actc,Stm542acag),oneSTSprimerandonePCRprimer(GAG5-6)linkedtoGli-B1andGlu-B3lociforselectionofy-gliadin45andLMW-2gluteninseitheralltogetheroralternatively.Asaresult,thestudywascompletedinthreeyearsinsteadofsixyearsrequiredinaclassicalbackcrossbreedingstudy,meaningabout50%timesaving,andobtainedahighqualitycandidatevariety.

【 授权许可】

Unknown