Distribution of Lipase Activity in Selected Tissues of Rainbow Trout (Oncorhynchus mykiss)

Journal of Applied Biological Sciences

Farzaneh NOORI¹  Nasser AGH¹  Aliasghar MOGHADDAM¹  Abdolali CHALECHALE¹  Isaac KARIMI¹  Amir DADYAN¹  Másume YOUSEFI¹ 

关键词: lipolytic activity;    pyloric caecae;    bile salt;    fish;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Lipases(EC3.1.1.3.)arewater-solubleenzymesthatcatalyzethehydrolysisofesterbondsinwater–insoluble,lipidsubstrates.Althoughthedistributionandpropertiesofvariouslipasesfrommanytissuesofhuman,laboratoryanddomesticanimalshavebeenstudied,littleisknownaboutthepatternofdistributionandphysiologicalrolesoftheseenzymesintherainbowtrout.Theaimofthepresentstudywastoexamineandcomparethedistributionoflipaseindifferenttissuesoftherainbowtrout.Aselectionoftissuesampleswasassayedforlipaseusingtheoliveoilsplittingmethodforthedeterminationofformedfreefattyacids.Significantactivityofthisenzymewasfoundthroughoutdigestivesystem,whichprovokesthatthepyloriccaecalmasshasthehighestspecificactivityoflipasefollowedbytheliver,kidney,stomach,spleen,distalintestine,proximalintestine,gill,heart,abdominalmuscleandbrain.Highactivityoflipaseinthepyloriccaecalmassofrainbowtroutindicatesitsprimaryroleinfatdigestion.However,itswidetissuedistributionsuggeststhatthisenzymemightperformotherfunctionsbesidegastrointestinalfatdigestion.Thelipaseactivityinpyloriccaecalmassseemstobedependenttobilesaltsunderourassayconditions,resultinginasignificantactivityinthepresenceofnaturalbovinebile.Theresultsofthisstudywillbediscussedintermsoftheinvolvementoflipaseinseveralbiochemicalandphysiologicalfunctionsinthisspecies.

【 授权许可】

Unknown