Production of Thermostable and Acidophilic Amylase from Thermophilic Bacillus licheniformis JAR-26

Journal of Applied Biological Sciences

Ram LAL¹  Atul KAUSHIK¹  Jeevan JYOTI¹  Nand LAL¹ 

关键词: Acidophilic amylase;    Bacillus sp.;    Liquefaction;    Thermostable;    Saccharification;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Thepresentstudyhasbeenconductedon120bacterialisolatescollectedfromspoiledstarchymaterialslikewheatflourandbreaddough,soilandspoiledsourmaterialsliketomatoes,citrusfruitsandpearsetc.Theseisolateswerescreenedfortheproductionofextracellularacidophilicandthermostableamylases.Fiveisolatesexhibitedgoodamylaseactivity.PreliminaryidentificationoftheseisolatesshowedthattheyallbelongtothegenusBacillus,sotheyweredesignatedasBacillussp.JAR-4,JAR-26,JAR-29,JAR-53andJAR-76.IsolateJAR-26showingmaximumstarchhydrolysiswasfurthersubjectedtobiochemicaltestingandonthebasisofresultsitwasdesignatedasBacilluslicheniformisJAR-26.Itwasabletogrowupto550CatpH5.5andshowedremarkableliquefactionaswellassaccharificationactivity.TheoptimumperiodformaximumcellgrowthandamylaseproductionincaseofBacillussp.JAR-26wasfoundtobe36hat450C.Theoptimumtemperatureforenzymeassaywas850CatpH5.5.Theenzymewasalsothermostableas100%activitywasobservedat850Cand55%activityretainedat1000Cand48%activityat1040Cwhenheatedfor30min.

【 授权许可】

Unknown