Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli

Journal of Applied Biological Sciences

SREEVIDHYA¹  Lawrance ANBU RAJAN¹  Yeragudipati RAJALAKSHMI¹  Vetrivel UMASHANKAR¹ 

关键词: trehalose-6-phosphate synthase;    trehalose-6-phosphate phosphatase;    compatible solutes;    salt stress;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Trehalose,anon-reducingdisaccharideisamajorcompatiblesolute,maintainsfluidityofmembranesandprotectsthebiologicalstructureoftheorganismsunderstress.Inthisstudy,trehalose-6-phosphatesynthase(TPS)andtrehalose-6-phosphatephosphatase(TPP)genesencodingfortrehalosebiosynthesiswerePCRamplifiedfromgenomicDNAofEscherichiacoliisolatedfromRohu(Labeorohita)collectedfromlocalfishmarketofCochin,Kerala,India.Theamplifiedgeneswereclonedandnucleotidesequencesweredetermined.Thesequencingresultsshowedthat(TPS)and(TPP)genescontain1,424bpand801bplongORFencoding474and266aminoacidsrespectively.Insilicosequenceanalysisrevealedthatthe(TPS)and(TPP)sequencesofE.coliwereconservedinmanyeubacteria.

【 授权许可】

Unknown