Risk Assessment of Food Coloring Agents on DNA Damage Using RAPD Markers

Journal of Applied Biological Sciences

Hala M. Abdelmigid¹ 

关键词: food colors;    RAPD;    DNA effects;    Vicia faba;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Inthisstudy,fababean(Viciafaba)seedlingswereusedasbioindicatortodeterminegenotoxiceffectofsyntheticdyescurrentlyusedasfoodcoloradditivesinmanycountries.Novelshort-termassaysarerequiredtosubstantiatethebatteryofassessmentmethodsforevaluatingthegenotoxicityofcandidatesubstances.Therefore,anattempthasbeenmadetoevaluaterandomlyamplifiedpolymorphicDNA(RAPD)analysisforitspotentialtoestablishgenotoxiceffectofcoloredfood.Forthepreliminaryassessment,thisstudycomparedtheeffectsoccurringatmolecularlevelsinViciafabaexposedtocoloredfoodatconcentrationsintherangeof0.2%to18.2%.ThequalitativemodificationsarisinginrandomamplifiedpolymorphicDNA(RAPD)profilesasameasureofDNAeffectswerecomparedwithcontrol.ResultssuggestedthattreatmentsoftheabovetestfoodsamplesreflectingchangesinRAPDprofiles.ChangesinRAPDpatternsincludedvariationinbandintensity;lossofnormalbandsandappearanceofnewbandscomparedwithcontrol.TheseresultsindicatedthatDNApolymorphismsdetectedbyRAPDanalysiscouldbeusedasaninvestigationtoolforenvironmentaltoxicologyandasausefulbiomarkerassayforthedetectionofgenotoxiceffectsoffooddyes.Inconclusion,themeasurementofparametersatmolecularlevelsisvaluableforinvestigatingthespecificeffectsofagentsinteractingwithDNA.Ultimately,thismethodologymayallowtheecotoxicologicalexaminationofthelinkbetweenmolecularalterationsandmeasurableadverseeffectsathigherlevelsofbiologicalorganization.

【 授权许可】

Unknown