Screening the Antimicrobial Activity of Biosurfactants Produced by Microorganisms Isolated from Refinery Wastewaters

Journal of Applied Biological Sciences

Aysun ERGENE¹  Emine YALÇIN¹ 

关键词: Biosurfactant;    antimicrobial activity;    FTIR spectrum;    refinery wastewater;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Inthisstudy,isolationandidentificationofmicroorganismsfrompetroleumrefinerywastewaterwithbiosurfactantproducingabilityandcharacterizationofbiosurfactantswereinvestigated.Biosurfactantproductionabilityofmicroorganismsisolatedfromwastewaterwasdeterminedbydropcollapsemethod.TheinitialcharacterizationofbiosurfactantswasinvestigatedbybiochemicaltestsandFTIRanalysis.Biosurfactantswereinvestigatedforpotentialantimicrobialactivityasinhibitionzone,minimuminhibitoryconcentration,andminimumbactericidalconcentrationagainstmedicallyimportantbacterialstrains.Wefoundthatthebiosurfactantsshowedprofoundlydistinctantimicrobialactivitytowardtestorganisms.MaximumantibacterialactivitywasshownbyBS-IIIagainstK.pneomoniaefollowedbyBS-IVagainstE.coli.Alsobiosurfactantsdisplayedaantifungalactivityagainsttestedyeastswiththediametersofzoneinhibitionrangingbetween12and17mm.ThemaximumantifungalactivitywasobtainedwithBS-IIIagainstC.krusei.TheMICvaluesobtainedinthisstudywerelowerthantheMBCvaluesforalltestedbiosurfactants.MaximumMBCvalueswererecordedwithE.coliandwere64mg/mlforBS-I.Themaincommercialuseofbiosurfactantsisoilindustry,foodsandenvironmentaltechnologybecauseoftheirabilitytostabilizeemulsions.Theantimicrobialactivityofbiosurfactantshasnotbeenreviewedextensively.Frompreliminarycharacterizationresultsitcanbesaidthatbiosurfactantsareasuitablealternativeinpotentialapplicationsofmedicalfields.

【 授权许可】

Unknown