Linkage mapping of Lentil (Lens culinaris L.) genome using recombinant inbred lines revealed by AFLP, ISSR, RAPD and some morphologic markers

Journal of Applied Biological Sciences

Fred MUEHLBAUER¹  Sehnaz OZATAY¹  Bahattin TANYOLAC¹  Abdullah KAHRAMAN¹ 

关键词: AFLP-RAPD-ISSR-Lentil-Segregation Distortion-Linkage map;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

WehaveconstructedamolecularlinkagemapofLentil(LensculinarisL.)inaRILpopulation(developedfromtheparentsPrecozxWA8949041)of94plantswith166markers.Theresultinglinkagemapconsistsof11(varyingbetween50.9–436.5cM)linkagegroupscovering1396.3cMwithanaveragemapdistancebetweenframeworkmarkersof8.4cM.MostoftheRAPDandISSRmarkerswereevenlydistributedacrossthegenome.Byusing40primercombinations,wewereabletogenerate391AFLPmarkersintheRILpopulation.ThemajorityoftheAFLPmarkersclusteredinlinkagegroup2.Inthesegregationdistortionanalysis,185markersdeviatedfromMendeliansegregation.Ofthe185markerswithskewedsegregation,108markerswereunlinkedandtherestofthedistortedmarkersmappedtolinkagegroups.LG2containedthemostdistortedmarkeramongthelinkagegroups.

【 授权许可】

Unknown