Effect of Melatonin on the Antioxidant Enzyme Status in the Liver of Swiss Albino Laboratory Mice in Single Circadian Rhythm

Journal of Applied Biological Sciences

İkbal AGAH İNCE,Zafer EREN,İrem GÜRKANLI¹ 

关键词: Melatonin;    Liver;    Antioxidant enzymes;    Superoxide dismutase;    Catalase;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Melatoninhasbeenshowntobeaneffectiveantioxidantinanumberofexperimentalmodelsbothinvitroandinvivo.However,limitedinformationisavailableonmonitoringtheeffectofmelatoninontheantioxidantenzymestatusintheliverinsinglecircadianrhythm.Tomonitortheeffectofmelatoninonantioxidantenzymeactivity,weusedSwissalbinoinbredmalemiceasamodelsystem.Thecrudeenzymefractionswerepreparedfromfreshlyisolatedliversat0,2,and24hoursaslightcycleand4,8,and12hoursasdarkcyclespecimens,postinjectionofmelatonin.TheinjectionofmelatoninresultedinastatisticallysignificantdecreaseinactivityofSODcomparedtocontrolgroup(p<0.01).However,harvestingtimedidnotaffectSODlevelssignificantly.TheactivityofCATwassignificantlydecreasedbymelatoninandthisdecreasewasclearlyrelatedtothetimeofharvest(p<0.01).InhibitiononSODactivitystartedatthebeginningofthedarkcycleofthecircadianrhythm,whileCATactivitystartedtodecreaseatthatpoint.TheinhibitionofCATreacheditsmaximumat8hourpostinjection.After12hourpostinjection,thebeginningofthelightcycleperiod;CATactivityturnedbacktothecontrollevel.Weconcludedthatbasedoncontroldata,internalmelatoninreleaseincreasesSODandCATactivity.Theexternalmelatonintreatmentdirectlyinhibitsmostly•OHradicalsandH2O2aswellassingletoxygen(¹O²).

【 授权许可】

Unknown