TILLING (Targetting Induced Local Lesions In Genomes) Technology for Plant Functional Genomics

Journal of Applied Biological Sciences

R. Soner SİLME¹  M. İlhan ÇAĞIRGAN¹ 

关键词: chemical mutagenesis;    reverse genetics;    single-nucleotide polymorphism discovery;    allelic series;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

ThepresentreviewdescribesTILLING(TargettingInducedLocalLesionsInGenomes)whichisageneralreversegeneticstrategythatworkswithamismatch-specificendonucleasetodetectinducedornaturalDNApolymorphismsingenesofinterest.Withhigh-throughputTILLING,rapidandlow-costdiscoveryofinducedpointmutationsinpopulationsofchemicallymutagenizedindividualsfromdiverseorganismsbecomespossible.Otheradvantagesareitsindependenceofgenomesize,reproductivesystemorgenerationtime.TILLINGyieldsatraditionalallelicseriesofpointmutationswhichmakeitvaluableforessentialgenes,wheresublethalallelesarerequiredforphenotypicanalysis.CurrentadvantagesofTILLINGhavemadeitanappropriatechoiceforthedetectionofbothinducedandnaturalvariationinseveralplantspecies.Here,wereviewrecentprogressinthistechnologyfortheresearchersofplantmutationanalysisandgenomicsera.

【 授权许可】

Unknown