INDUCED CHEMICAL MUTATION IN

Electronic journal of Polish agricultural universities

Krzysztof Wraga¹  Zuzanna Kantarek²  Marcelina KrupaMa³kiewicz² 

[1] Study of Ornamental Plants, West Pomeranian University of Technology in Szczecin, Poland;Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology in Szczecin, Poland

关键词: mutants;    DNA;    EMS;    ISSR analyssis;    liatria;   

DOI  :  

来源: Polish Agricultural Schools of Higher Education

【 摘 要 】

TheobjectiveofthepresentedstudywastoinducedmutationintwoliatrisspeciesL.ligulistylisandL.pycnostachyausingethylmethane-sulfonate(EMS)ataconcentrationof2.0and5.0mMandcharacterizationoftheobtainedmutants.Theresultingphenotypicchangesobservedinliatrisweremainlyrelatedtoanthocyanindiscolorationonshootsandinflorescencepetioles,changesintheanatomyofinflorescencepetiolesandleaves,aswellastheunevenrisingofinflorescencebuds.TheEMSmutagenconcentrationsusedintheexperimentrevealedaninhibitoryeffectonfeaturessuchastheheightandwidthofplants,thecircumferenceandweightofbulbsandthenumberofbudsonthebulb.Itwasfoundthat,thefrequencyofallobservedphenotypicchangesinL.ligulistylisandL.pycnostachyawashigheraftertheapplicationof2.0mMEMSsolution(11and3%,respectively).FortheassessmentofgenotypicchangesattheDNAleveltheintersimplesequencerepeat(ISSR)markerswereadopted.BasedonthedendrogramproducedfromastudyanalysisofthesimilaritycoefficientsusingUPGMAitwasfoundthatthemutantsunderstudyshoweda43.5ndash;75.7%similaritytoeachother.

【 授权许可】

Unknown