Genetic polymorphism and haplotypes of HLA-A, -B, and -DRB1 locus distribution analysis based on low-resolution HLA-typing of umbilical cord blood samples stored in the public bank of the North-West region of the Russian Federation

Cellular Therapy and Transplantation

Svetlana A. Smirnova¹  Ekaterina S. Kudryavtseva¹  Ksenia V. Shunkina¹  Elena A. Kotelevskaya¹  Alexander B. Smolyaninov¹ 

关键词: hematopoietic stem cells;    umbilical cord blood;    cord blood public register;    transplantation;    HLA-typing;   

DOI  :  10.3205/ctt-2011-No12-abstract86

学科分类：肿瘤学

来源: Universitaetsklinikum Hamburg - Eppendorf / University Medical Center Hamburg - Eppendorf

【 摘 要 】

Aim:TheaimofthegiveninvestigationistodeterminethemajorhistocompatibilitycomplexantigensofUCBsamplesfromapublicbankbyamolecular-geneticmethod,basedonthequot;StemCellBankPokrovskyquot;,andalsotoestablishalistofsamplesandtoanalyzelociHLA-A,-B,-DRB1polymorphismoftheresidentsoftheNorth-WestregionofRussia.Methods:Duringthestudy,430samplesofcordbloodwereanalyzedusingpolymerasechainreaction(PCR)withsequencespecificprimers(SSP).DNAwasisolatedfrom0.7mlofwholebloodbyDNAextractioncolumnProtransDNABox500(Protrans,Germany).HLA-A,HLA-B,andHLA-DRB1lociwereanalyzedusingthecyclerplatesystemProtransHLA-A*,-B*,-DRB1*(Protrans,Germany)andBiotest(BiotestMedicalDiagnostics,Germany)accordingtothemanufacturer’sinstructions.Results:Itwasidentifiedthatthemostfrequentallelesare:HLA-A*02,28.1%;*03,14.5%;*24,14.0%;HLA-B*07,13.3%;*35.12.7%;*44,8.2%;HLA-DRB1*15,15.8;*13,15.0%;andnbsp;*03,12.5%.Conclusions:TheseresultsofgeneticpolymorphismofHLA-A,-B,-DRB1locidistributionintheseinhabitantsoftheNorth-WestregionofRussiacancomplementtheexistinginformationdatabaseonpopulationgeneticsandassociateddiseases.ThemainadvantageofowningourownpublicbankofUCBsamplesintheNorth-WestregionofRussia—inadditiontoreducingthecostandavailabilityofmaterialfortransplantation—isthattheprobabilityofmatchingHLA-genotypesofonepopulationismuchhigherthanfindingthemthroughaglobalregister.

【 授权许可】

Unknown