The telomere length of cord blood cells as an additional characteristic of transplant quality

Cellular Therapy and Transplantation

Natalia V. Smirnova¹  Olga V. Supilnikova¹  Alexandrina S. Khrupina¹  Alexander B. Smolyaninov¹  Dmitrii A. Ivolgin¹  Polina Yu. Novikova¹  Svetlana Yu. Novikova¹ 

关键词: telomere;    flow-FISH;    cord blood cells;    sample quality;   

DOI  :  10.3205/ctt-2010-No9-abstract35

学科分类：肿瘤学

来源: Universitaetsklinikum Hamburg - Eppendorf / University Medical Center Hamburg - Eppendorf

【 摘 要 】

TelomeresaretheendsofthechromosomesandrepresentrepeatedDNAsequencesandproteincomplexes.Telomeresshortenwitheachcelldivision,whichlimitstheproliferationpotentialofcells.Currentlythereisintensivedevelopmentintheclinicalapplicationoftelomerelength.Additionalcharacteristicsofstemcellproliferationactivitybytelomerelengthcanbeaninformativeindicatoroftransplantquality.Weanalyzed15cordbloodsamplesusingflow-FISH,ELISA,hematologicalanalyzer,andnumberofCD34+CD45dimcells.TheaveragetelomerelengthofCBcells’leukocytesfractionwas20.1±4.8%respectiveto1301controlcells(T-celllymphoblasticleukemia).LinearcorrelationanalysisdidnotfindanyconnectionbetweenthetelomerelengthoftheCBcells’leukocytefraction,andCD34+CD45dimcellnumbers(r=-0.49),whichisinlinewithpublisheddata.However,theaveragetelomerelengthoftheCBleukocytefractionpositivelycorrelateswithmonocyteconcentration(r=0.65).Therewerecomparativelylongtelomeres(23.6and23.9%)intwosamplesoftheCBcell’leukocytefractionwithoutanycontamination.Thisdataconfirmsthattheflow-FISHmethodissuitablefortheestimationoftelomerelengthoftheleukocytefractionofCBcells.AveragetelomerelengthoftheleukocytefractionofCBcellsobliquelyshowsCBsamplequality,duetoapositivecorrelationwithmonocyteconcentrationandcontaminationabsence.

【 授权许可】

Unknown