Cellular Therapy and Transplantation | |
Minimal residual disease evaluation in patients with acute lymphoblastic leukemia | |
Elena V. Babenko1  Elena V. Semenova1  Evgeniy G. Shumilov1  Alexandra A. Sipol1  Boris V. Afanasyev1  | |
关键词: allogeneic hematopoietic stem cell transplantation; acute lymphoblastic leukemia; minimal residual disease; flow cytometry; | |
DOI : 10.3205/ctt-2010-No9-abstract11 | |
学科分类:肿瘤学 | |
来源: Universitaetsklinikum Hamburg - Eppendorf / University Medical Center Hamburg - Eppendorf | |
【 摘 要 】
Minimalresidualdisease(MRD)isthepersistenceofalownumberofleukemiccellsinpatientswhoachieveclinicalandhematologicalremission.TheaimofthisstudywastodefinetheprognosticvalueofMRDdetectionbyflowcytometry.nbsp;nbsp;Materialsandmethods:Weanalyzed145bonemarrowsamplesfrom41patientswithmultiparametryflowcytometry.21patients(childrenandyoungadults)hadnewlydiagnosedB-precursorsleukemia,treatedwiththechemotherapyprotocol“Moscow-Berlin2008â€,and20patientshadundergoneallogeneichematopoieticstemcelltransplantation(alloHSCT).Thefollowingmonoclonalantibodycombinationswereused:CD10/CD34/CD19/CD20,CD22/CD34/CD19/CD45,CD38/CD34/CD19/CD45.ToverifytheirMRD-statuswecomparedtheflowcytometrydatawithmolecularbiology,cytogenetic,andmorphologyassays.Results:AttheendoffirstconsolidationtherapyweobservedapositiveMRDin4patients(19%);threeofthemalsohadapositiveMRDafterthesecondconsolidation,whichthenbecamenegative.nbsp;nbsp;nbsp;nbsp;Fourpatients(19%)hadapositiveMRDstatusafterthesecondconsolidation.Allpatientswereinremissionatthetimeofthestudy(medianofobservation11months).In10patients(50%)post-alloHSCTtheMRDwasnegative,whichcorrespondedtothemolecularbiologydata.ThreepatientshadonepositiveMRD,theyalsostayedinremission.Fourpatients(20%)hadMRD-positivestatusoneverytime-pointofobservation.Threeofthemdevelopedrelapse.Overall4-yearsurvivalwassignificantlyhigher(p=0.014)inpatientswithanegativeMRDstatusoneverytime-point.nbsp;nbsp;nbsp;Conclusion:MeasurementofMRDbymultiparametryflowcytometry,basedonleukemia-associatedimmunophenotype,providesprognosticinformationinchildrenwithALL.
【 授权许可】
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【 预 览 】
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