Minimal residual disease evaluation in patients with acute lymphoblastic leukemia

Cellular Therapy and Transplantation

Elena V. Babenko¹  Elena V. Semenova¹  Evgeniy G. Shumilov¹  Alexandra A. Sipol¹  Boris V. Afanasyev¹ 

关键词: allogeneic hematopoietic stem cell transplantation;    acute lymphoblastic leukemia;    minimal residual disease;    flow cytometry;   

DOI  :  10.3205/ctt-2010-No9-abstract11

学科分类：肿瘤学

来源: Universitaetsklinikum Hamburg - Eppendorf / University Medical Center Hamburg - Eppendorf

【 摘 要 】

Minimalresidualdisease(MRD)isthepersistenceofalownumberofleukemiccellsinpatientswhoachieveclinicalandhematologicalremission.TheaimofthisstudywastodefinetheprognosticvalueofMRDdetectionbyflowcytometry.nbsp;nbsp;Materialsandmethods:Weanalyzed145bonemarrowsamplesfrom41patientswithmultiparametryflowcytometry.21patients(childrenandyoungadults)hadnewlydiagnosedB-precursorsleukemia,treatedwiththechemotherapyprotocol“Moscow-Berlin2008”,and20patientshadundergoneallogeneichematopoieticstemcelltransplantation(alloHSCT).Thefollowingmonoclonalantibodycombinationswereused:CD10/CD34/CD19/CD20,CD22/CD34/CD19/CD45,CD38/CD34/CD19/CD45.ToverifytheirMRD-statuswecomparedtheflowcytometrydatawithmolecularbiology,cytogenetic,andmorphologyassays.Results:AttheendoffirstconsolidationtherapyweobservedapositiveMRDin4patients(19%);threeofthemalsohadapositiveMRDafterthesecondconsolidation,whichthenbecamenegative.nbsp;nbsp;nbsp;nbsp;Fourpatients(19%)hadapositiveMRDstatusafterthesecondconsolidation.Allpatientswereinremissionatthetimeofthestudy(medianofobservation11months).In10patients(50%)post-alloHSCTtheMRDwasnegative,whichcorrespondedtothemolecularbiologydata.ThreepatientshadonepositiveMRD,theyalsostayedinremission.Fourpatients(20%)hadMRD-positivestatusoneverytime-pointofobservation.Threeofthemdevelopedrelapse.Overall4-yearsurvivalwassignificantlyhigher(p=0.014)inpatientswithanegativeMRDstatusoneverytime-point.nbsp;nbsp;nbsp;Conclusion:MeasurementofMRDbymultiparametryflowcytometry,basedonleukemia-associatedimmunophenotype,providesprognosticinformationinchildrenwithALL.

【 授权许可】

Unknown