Journal of Oral Microbiology | |
The impact of virulence factors ofPorphyromonas gingivalison wound healingin vitro | |
1  Alexa M. G. A. Laheij2  | |
[1] hannes J. de Soet;Academic Center for Dentistry Amsterdam, University of Amsterdam and VU University, Amsterdam, The Netherlands | |
关键词: gingipains; capsular polysaccharide; P. gingivalis; LPS; cell migration essay; oral epithelial cells; re-epithelialization; | |
DOI : | |
来源: Co-Action Publishing | |
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【 摘 要 】
Background:Porphyromonasgingivalisinhibitsoralepithelialwoundhealinginvitromorestronglythanotheroralbacteria,butitisunknownwhyP.gingivalisissuchapotentinhibitorofwoundhealing.Objective:Therefore,theaimofthisstudywastoinvestigatetheinfluenceofmajorvirulencefactorsofP.gingivalisonwoundhealinginaninvitrowound-healingmodel.Theinfluenceofthecapsularpolysaccharide,theArg-andLys-gingipains,themajorfimbriaeandlipopolysaccharide(LPS)wasinvestigated.Design:AstandardizedscratchwasmadeinaconfluentlayerofhumanoralepithelialcellsHO-1-N-1.TheepithelialcellswerethenchallengedwithdifferentconcentrationsofseveralP.gingivaliswild-typestrainsandknockoutmutants.Closureofthescratchwasdeterminedafter17handcomparedtocontrolconditionswithoutbacteria.Results:TheP.gingivalisstrainsATCC33277,W83,andW50significantlyinhibitedwoundhealing.Thepresenceofacapsularpolysaccharideloweredsignificantlytheinhibitionofepithelialcellmigration,whilegingipainactivitysignificantlyincreasedtheinhibitionofcellmigration.LPSandthemajorfimbriaedidnotinfluenceepithelialcellmigration.NoneofthetestedP.gingivalisstrainscompletelypreventedtheinhibitionofcellmigration,suggestingthatothercharacteristicsofP.gingivalisalsoplayaroleintheinhibitionofwoundhealing,andthatfurtherresearchisneeded.Conclusions:ThecapsularpolysaccharideandtheArg-andLys-gingipainsofP.gingivalisinfluencedthecapacityofP.gingivalistohinderwoundhealing,whileLPSandthemajorfimbriaehadnoeffect.Keywords:gingipains;capsularpolysaccharide;P.gingivalis;LPS;cellmigrationessay;oralepithelialcells;re-epithelialization(Published:2July2015)Citation:JournalofOralMicrobiology2015,7:27543-http://dx.doi.org/10.3402/jom.v7.27543
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