The impact of virulence factors ofPorphyromonas gingivalison wound healingin vitro

Journal of Oral Microbiology

¹  Alexa M. G. A. Laheij² 

[1] hannes J. de Soet;Academic Center for Dentistry Amsterdam, University of Amsterdam and VU University, Amsterdam, The Netherlands

关键词: gingipains;    capsular polysaccharide;    P. gingivalis;    LPS;    cell migration essay;    oral epithelial cells;    re-epithelialization;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Background:Porphyromonasgingivalisinhibitsoralepithelialwoundhealinginvitromorestronglythanotheroralbacteria,butitisunknownwhyP.gingivalisissuchapotentinhibitorofwoundhealing.Objective:Therefore,theaimofthisstudywastoinvestigatetheinfluenceofmajorvirulencefactorsofP.gingivalisonwoundhealinginaninvitrowound-healingmodel.Theinfluenceofthecapsularpolysaccharide,theArg-andLys-gingipains,themajorfimbriaeandlipopolysaccharide(LPS)wasinvestigated.Design:AstandardizedscratchwasmadeinaconfluentlayerofhumanoralepithelialcellsHO-1-N-1.TheepithelialcellswerethenchallengedwithdifferentconcentrationsofseveralP.gingivaliswild-typestrainsandknockoutmutants.Closureofthescratchwasdeterminedafter17handcomparedtocontrolconditionswithoutbacteria.Results:TheP.gingivalisstrainsATCC33277,W83,andW50significantlyinhibitedwoundhealing.Thepresenceofacapsularpolysaccharideloweredsignificantlytheinhibitionofepithelialcellmigration,whilegingipainactivitysignificantlyincreasedtheinhibitionofcellmigration.LPSandthemajorfimbriaedidnotinfluenceepithelialcellmigration.NoneofthetestedP.gingivalisstrainscompletelypreventedtheinhibitionofcellmigration,suggestingthatothercharacteristicsofP.gingivalisalsoplayaroleintheinhibitionofwoundhealing,andthatfurtherresearchisneeded.Conclusions:ThecapsularpolysaccharideandtheArg-andLys-gingipainsofP.gingivalisinfluencedthecapacityofP.gingivalistohinderwoundhealing,whileLPSandthemajorfimbriaehadnoeffect.Keywords:gingipains;capsularpolysaccharide;P.gingivalis;LPS;cellmigrationessay;oralepithelialcells;re-epithelialization(Published:2July2015)Citation:JournalofOralMicrobiology2015,7:27543-http://dx.doi.org/10.3402/jom.v7.27543

【 授权许可】

Unknown