Extended-spectrum beta-lactamase-producing bacteria are not detected in supragingival plaque samples from human fecal carriers of ESBL-producingEnterobacteriaceae

Journal of Oral Microbiology

P氓l Arne Jenum¹  Arnfinn Sundsfjord⁴  Ingar Olsen²  Ola Bj酶rang³  Trude Handal²  Arne S酶raas⁵ 

[1] Department of Medical Microbiology, Vestre Viken Hospital Trust, B忙rum, Norway;Department of Oral Biology, University of Oslo, Oslo, Norway;Department of Medical Microbiology, Vestre Viken Hospital Trust, B忙rum, Norway;Department of Microbiology and Infection Control, Reference Centre for Detection of Antimicrobial Resistance, University Hospital of North Norway, Troms酶, Norway, and Department of Medical Biology, Research Group for Host-Microbe Interactions, Faculty of Health Sciences, University of Troms酶, Troms酶, Norway;Department of Medical Microbiology, Vestre Viken Hospital Trust

关键词: Gram-negative bacteria;    Transmission;    Carriage;    Extended spectrum betalactamase;    Epidemiology;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Background:TheprevalenceofinfectionscausedbyCefotaximase-Munich(CTX-M)-typeextended-spectrumbeta-lactamase-producingEnterobacteriaceae(ESBL-E)hasrapidlyincreasedduringthepast15years.Enterobacteriaceaearecommonlyfoundinthegastrointestinaltractandlong-termintestinalcarriageisconsideredimportantforthespreadofESBLandasasourceofclinicalinfections.OralbiofilmsuchassupragingivalplaqueisknowntocontainnumerousantibioticresistancedeterminantsandmayalsorepresentapoorlyinvestigatedsiteforESBLcarriageandfurtherspread.Objective:ToinvestigatepossiblecarriageofESBL-producingbacteriainsupragingivalplaqueofknownfecalcarriersofthesebacteria.Design:WescreenedforthepresenceofaerobicandanaerobicESBL-producingbacteriaandblaCTX-Minsupragingivalplaquesamplesfromhealthyhumanadultswithculture-verifiedfecalcarriageofCTX-M-producingEscherichiacoli.ThepresenceorabsenceofEnterobacteriaceaeandESBL-producingbacteriainplaquesampleswasevaluatedusingculture-basedmethodsandconsensusCTX-MPCR.Results:Oralsampleswereobtainedfrom17participantswithknownpreviouscarriageofESBL-producingE.coli.NoESBL-producingbacteriaorESBLgenesweredetectedusingculture-basedandmolecularmethods.OnecolonyofRahnellaaquatilisharboringtheclassAESBLgeneblaRAHN-1/2wasidentifiedinanoralsamplefromoneoftheparticipants.Conclusion:ThispilotstudysupportsthenotionthatthepresenceofCTX-M-producingbacteriaisuncommoninoralplaqueofhealthyhumanadultfecalcarriers.Duetothelimitednumberofpersonstested,alowprevalenceoforalESBL-carriageinhealthyadultsorcarriageinselectedgroupsofpatientscannotbeexcluded.Toourknowledge,thisisthefirstdescriptionofanR.aquatiliswiththeRAHN-1/2geneintheoralcavity.Keywords:CTX-M;oralcarriage;extended-spectrum尾-lactamase;Enterobacteriaceae(Published:20August2014)Citation:JournalofOralMicrobiology2014,6:24026-http://dx.doi.org/10.3402/jom.v6.24026ResponsibleEditor:Clay聽B.聽Walker,UniversityofFlorida,USA.*TheChiefEditorIngarOlsen,hasnothadanypartinthereviewanddecisionprocessforthispaper.

【 授权许可】

Unknown