Characterization of extracellular polymeric matrix, and treatment ofFusobacterium nucleatumandPorphyromonas gingivalisbiofilms with DNase I and proteinase K

Journal of Oral Microbiology

Marwan Mansoor Ali Mohammed¹  Audun H. Nerland¹  Mohammed Al-Haroni²  Vidar Bakken³ 

[1] The Gade Institute 鈥?Section for Microbiology and ImmunologyFaculty of Medicine and DentistryUniversity of Bergen;Department of Pharmacy, Faculty of Health Sciences, University of Troms酶, Troms酶, Norway.;The Gade Institute 鈥?Section for Microbiology and ImmunologyFaculty of Medicine and DentistryUniversity of Bergen

关键词: Subgingival biofilm;    extracellular polymeric matrix;    Fusobacterium nucleatum;    Porphyromonas gingivalis;    static and dynamic biofilm models;    confocal laser scanning microscopy (CLSM);   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Background:Biofilmsareorganizedcommunitiesofmicroorganismsembeddedinaself-producedextracellularpolymericmatrix(EPM),oftenwithgreatphylogeneticvariety.Bacteriainthesubgingivalbiofilmarekeyfactorsthatcauseperiodontaldiseases;amongthesearetheGram-negativebacteriaFusobacteriumnucleatumandPorphyromonasgingivalis.TheobjectivesofthisstudyweretocharacterizethemajorcomponentsoftheEPMandtotesttheeffectofdeoxyribonucleaseI(DNaseI)andproteinaseK.Methods:F.nucleatumandP.gingivalisbacterialcellsweregrownindynamicandstaticbiofilmmodels.TheeffectsofDNaseIandproteinaseKenzymesonthemajorcomponentsoftheEPMweretestedduringbiofilmformationandonmaturebiofilm.Confocallaserscanningmicroscopywasusedinobservingbiofilmstructure.Results:Proteinsandcarbohydrateswerethemajorcomponentsofthebiofilmmatrix,andextracellularDNA(eDNA)wasalsopresent.DNaseIandproteinaseKenzymeshadlittleeffectonbiofilmsintheconditionsused.Intheflowcell,F.nucleatumwasabletogrowinpartiallyoxygenatedconditionswhileP.gingivalisfailedtoformbiofilmaloneinsimilarconditions.F.nucleatumsupportedthegrowthofP.gingivaliswhentheyweregrowntogetherasdualspeciesbiofilm.Conclusion:DNaseIandproteinaseKhadlittleeffectonthebiofilmmatrixintheconditionsused.F.nucleatumformedbiofilmeasilyandsupportedthegrowthofP.gingivalis,whichpreferredanaerobicconditions.Keywords:Subgingivalbiofilm;extracellularpolymericmatrix;Fusobacteriumnucleatum;Porphyromonasgingivalis;staticanddynamicbiofilmmodels;confocallaserscanningmicroscopy(Published:29Janaury2013)Citation:JournalofOralMicrobiology2013,5:20015-http://dx.doi.org/10.3402/jom.v5i0.20015

【 授权许可】

Unknown