Antibiotic susceptibility ofAggregatibacter actinomycetemcomitansJP2 in a biofilm

Journal of Oral Microbiology

Orit Oettinger-Barak²  Stuart G. Dashper²  Deanne V. Catmull²  Geoffrey G. Adams²  Michael N. Sela⁴  Eli E. Machtei³  Eric C. Reynolds¹ 

[1] Melbourne Dental School, Oral Health CRC, Bio21 Institute, The University of Melbourne.;Melbourne Dental School, Oral Health CRC, Bio21 Institute, The University of Melbourne.;Dept. of Periodontology, School of Graduate Dentistry, Rambam Health Care Campus, Haifa, Israel;Laboratory of Oral Microbiology, Faculty of Dental Med, Hebrew University, Jerusalem, Israel

关键词: Localized Aggressive Periodontitis;    Aggregatibacter actinomycetemcomitans;    doxycycline;    metronidazole;    biofilm;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Background:Localizedaggressiveperiodontitis(LAgP)isaninflammatorydiseaseassociatedwithspecificbacteria,particularlyAggregatibacteractinomycetemcomitans,whichcanresultinearlytoothloss.Thebacteriagrowasabiofilmknownassubgingivalplaque.Treatmentincludesmechanicaldebridementofthebiofilm,oftenassociatedwithempiricalantibiotictreatment.Objective:TheaimsofthisstudyweretotestinvitrothesensitivityofA.actinomycetemcomitansJP2duringplanktonicandbiofilmgrowthtodoxycyclineandtothecombinationofmetronidazoleandamoxicillin,whicharetwoantibioticprotocolscommonlyusedinclinicalpractice.Design:Twoinvitrobiofilmmodelswereusedtotesttheeffectsoftheantibiotics:astatic96-wellplateassaywasusedtoinvestigatetheeffectoftheseantibioticsonbiofilmformationwhilstaflowchambermodelwasusedtoexaminetheeffectonestablishedbiofilms.Results:Oftheantibioticstestedinthismodelsystem,doxycyclinewasmostefficaciouswithaminimalinhibitoryconcentration(MIC)againstplanktoniccellsof0.21mg/Landminimalbiofilminhibitoryconcentration(MBIC)of2.10mg/L.Themostcommonlyprescribedantibioticregimen,amoxicillinmetronidazole,wasmuchlesseffectiveagainstbothplanktonicandbiofilmcellswithanMICandMBICof12.0mg/Land20.2mg/L,respectively.Asingletreatmentoftheclinicallyachievableconcentrationof10mg/LdoxycyclinetosparseA.actinomycetemcomitansbiofilmsintheflowchambermodelresultedinsignificantdecreasesinbiofilmthickness,biovolume,andcellviability.DenseA.actinomycetemcomitansbiofilmsweresignificantlymoreresistanttodoxycyclinetreatment.Lowconcentrationsofantibioticsenhancedbiofilmformation.Conclusion:A.actinomycetemcomitansJP2homotypicbiofilmsweremoresusceptibleinvitrotodoxycyclinethanamoxicillinmetronidazole.Keywords:Localizedaggressiveperiodontitis;Aggregatibacteractinomycetemcomitans;doxycycline;metronidazole;biofilm(Published:8May2013)Citation:JournalofOralMicrobiology2013,5:20320-http://dx.doi.org/10.3402/jom.v5i0.20320

【 授权许可】

Unknown