Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients

Journal of Oral Microbiology

Elaine M. Haase¹  Seok-Mo Heo²  Frank A. Scannapieco²  Robert S. Sung² 

[1] University at Buffalo;University at Buffalo

关键词: Oral microbiology;    epidemiology;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

0false18pt18pt00falsefalsefalseCandidaalbicansoftenresidesintheoralcavityofhealthyhumansasaharmlesscommensalorganism.Thisopportunisticfunguscancausesignificantdiseaseincriticallyillpatients,suchasthoseundergoingmechanicalventilationintheintensivecareunit(ICU)havingcompromisedlocalairwaydefensemechanisms.Thegoalofthisstudywastodeterminetheintra-andinter-patientgeneticrelationshipbetweenstrainsofC.albicansrecoveredfromdentalplaque,trachealsecretions,andthelowerairwaybybronchoalveolarlavageofpatientsundergoingmechanicalventilation.Threepulsed-fieldgelelectrophoresis(PFGE)typingmethodswereusedtodeterminethegeneticrelatednessoftheC.albicansstrains,includingelectrophoretickaryotyping(EK)andrestrictionendonucleaseanalysisofthegenomeusingSfiI(REAG-S)andBssHII(REAG-B).TheC.albicansisolatesfromdentalplaqueandtracheo-bronchialsitesfromthesamepatientweregeneticallyindistinguishableandretainedovertime,whereasstrainsfromdifferentpatientsusuallyseparatedintodifferentgenotypes.Amongthethreemethods,REAG-Bprovedtobethemostdiscriminatorymethodtodifferentiateisolates.Thefindingofgeneticallysimilarstrainsfromtheoralandtracheo-bronchialsitesfromthesamepatientsupportsthenotionthattheoralcavitymayserveasanimportantsourceforC.albicansspreadtothetracheaandlungofmechanicallyventilatedpatients.Keywords:yeast;pulsed-fieldgelelectrophoresis(PFGE);molecularepidemiology;mechanicalventilation;oralcavity(Published:20June2011)Citation:JournalofOralMicrobiology2011,3:6362-DOI:10.3402/jom.v3i0.6362

【 授权许可】

Unknown