Identification of interspecies interactions affectingPorphyromonas gingivalisvirulence phenotypes

Journal of Oral Microbiology

Wai S. Cheung²  Elizabeth L. Tenorio¹  Linden T. Hu³  Brian A. Klein¹ 

[1] Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center;Department of Periodontology, Tufts School of Dental Medicine;Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center

关键词: oral microbiology;    periodontitis;    virulence factors;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Background:Periodontitisisrecognizedasacomplexpolymicrobialdisease,however,theimpactofthebacterialinteractionsamongthe700-1,000differentspeciesoftheoralmicrobiotaremainspoorlyunderstood.Weconductedaninvitroscreenfororalbacteriathatmitigateselectedvirulencephenotypesoftheimportantperiodontalpathogen,Porphyromonasgingivalis.Methods:Weisolatedandidentifiedoralanaerobicbacteriafromsubgingivalplaqueofdentalpatients.WhencoculturedwithP.gingivalisW83,specificisolatesreducedthecytopathogeniceffectsofP.gingivalisonoralepithelialcells.Results:Inaninitialscreenof103subgingivalisolates,weidentified19distinctstrainsfromninespeciesofbacteria(includingActinomycesnaeslundii,Streptococcusoralis,Streptococcusmitis,andVeilonelladispar)thatprotectoralepithelialcellsfromP.gingivalis-inducedcytotoxicity.WefoundthatsomeofthesestrainsinhibitedP.gingivalisgrowthinplateassaysthroughtheproductionoforganicacids,whereassomedecreasedthegingipainactivityofP.gingivalisincocultureormixingexperiments.Conclusion:Insummary,weidentified19strainsisolatedfromhumansubgingivalplaquethatinteractedwithP.gingivalis,resultinginmitigationofitscytotoxicitytooralepithelialcells,inhibitionofgrowth,and/orreductionofgingipainactivity.UnderstandingthemechanismsofinteractionbetweenbacteriaintheoralmicrobialcommunitymayleadtothedevelopmentofnewprobioticagentsandnewstrategiesforinterruptingthedevelopmentofperiodontaldiseaseKeywords:oralpathogen;bacterialinteractions;virulence;gingipain;cytotoxicity(Published:17October2011)Citation:JournalofOralMicrobiology2011,3:8396-DOI:10.3402/jom.v3i0.8396

【 授权许可】

Unknown