Nitric oxide release from trigeminal satellite glial cells is attenuated by glial modulators and glutamate

International Journal of Physiology, Pathophysiology and Pharmacology

Rishi Kumar Somvanshi¹  Xu-Dong Dong¹  Parisa Gazerani¹  Jens Christian Laursen¹  Ujendra Kumar¹  Brian Edwin Cairns¹  Lars Arendt-Nielsen¹ 

[1] Center for Sensory-Motor Interaction, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Fredrik Bajers Vej 7D3, Aalborg Ø, DK-9220, Denmark; Faculty of Pharmaceutical Sciences, The University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC, Canada V6T 1Z3; College of Stomatology, Tianjin Medical University, Tianjin 300071, China

关键词: Ibudilast;    vitamin D3;    migraine;    headache;    satellite glial cells;    nitric oxide;    glutamate;    glial modulation;   

DOI  :  

学科分类：生理学与病理学

来源: e-Century Publishing Corporation

【 摘 要 】

Nitricoxide(NO)issuggestedtoplayanimportantroleinprimaryheadaches.IthasbeenproposedthatreleaseofNOfromsatelliteglialcells(SGCs)ofthetrigeminalganglion(TG)couldcontributetothepathogenesisoftheseheadaches.TheprincipalaimofthisstudywastoinvestigateifthephosphodiesteraseinhibitorIbudilast(Ibu)and1#945;,25-dihydroxyvitaminD3(VitD3)couldinterferewithNOreleasefromtrigeminalSGCs.SinceglutamateisreleasedfromactivatedTGneurons,theabilityofglutamatetoalterNOreleasefromSGCswasalsoinvestigated.Tostudythis,weisolatedSGCsfromtheTGofadultmaleSprague-Dawleyrats,provokedNOreleasefromSGCswithforskolin(FSK;0.1,1,10#181;M),andexaminedtheeffectofgradedconcentrationsofIbu(1,10,100#181;M),VitD3(5,50,500nM),andglutamate(10,100,1000#181;M).OurresultsindicatethatbothIbuandVitD3arecapableofattenuatingtheFSK-mediatedincreasedNOreleasefromSGCsafter48hoursofincubation.Lowerglutamateconcentrations(10and100#181;M)significantlydecreasedNOreleasenotonlyunderbasalconditionsafter24and48hours,butalsoafterSGCswerestimulatedwithFSKfor48hours.Inconclusion,NOreleasefromSGCsharvestedfromtheTGcanbeattenuatedbyglialmodulatorsandglutamate.AsNOisthoughttoincreaseTGneuronexcitability,thefindingssuggestthattargetingSGCsmayprovideanoveltherapeuticapproachformanagementofcraniofacialpainconditionssuchasmigraineinthefuture.(IJPPP1310001).

【 授权许可】

Unknown