Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source

Chemical and biochemical engineering quarterly

J. Chu¹  X. Zou¹  Y. Zhuang¹  H. Hang¹  S. Zhang¹  C. Chen¹ 

关键词: Biological nitrogen;    erythromycin production;    fed-batch strategy;    Medium optimization;    response surface methodology;    Saccharopolyspora erythraea;   

DOI  :  

来源: Croatian Society of Chemical Engineers

【 摘 要 】

Abstract A novel source of biological nitrogen produced by multi-strain fermentation was developed for erythromycin production.The nitrogen composition of corn steep liquor, biological nitrogen and soybean flour was optimized by using Response surface methodology (RSM) firstly.A Box-Behnken design was used to estimate the optimum nitrogen composition for the production of erythromycin as follows: corn steep liquor: γ = 5.1 g L–1, biological nitrogen γ = 5.96 g L–1 and soybean flour γ = 24.17 g L–1. Based on the optimum nitrogen source, the constant glucose fed-batch and pH control fed-batch strategies were used to further optimize the erythromycin production in 50 L stirred bioreactor, respectively.It was found that pH control could serve as an effective control strategy for erythromycin production, and the maximum erythromycin production was 8528 U mL–1 at 190 h.This work demonstrates that the new medium formulation based on a cheap nitrogen source, biological nitrogen, is a potential alternative for economic erythromycin production on a large scale.

【 授权许可】

Unknown