De novo transcriptome sequencing in Frankliniella occidentalis to identify genes involved in plant virus transmission and insecticide resistance

Genomics

Weidi Li¹  Jian Yang¹  Pengjun Zhang¹  Jinming Zhang¹  Yawei Bei¹  Yaobin Lu¹  Zhijun Zhang¹  Fang Huang¹ 

关键词: Frankliniella occidentalis;    Tomato spotted wilt virus;    RNA-seq;    Transcriptome;    Illumina;   

DOI  :  10.1016/j.ygeno.2013.02.005

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

Thewesternflowerthrips(WFT),Frankliniellaoccidentalis,aworld-wideinvasiveinsect,causesagriculturaldamagebydirectlyfeedingandbyindirectlyvectoringTospoviruses,suchasTomatospottedwiltvirus(TSWV).WecharacterizedthetranscriptomeofWFTandanalyzedglobalgeneexpressionofWFTresponsetoTSWVinfectionusingIlluminasequencingplatform.Wecompiled59,932unigenes,andidentified36,339unigenesbysimilarityanalysisagainstpublicdatabases,mostofwhichwereannotatedusinggeneontology(GO)andKyotoEncyclopediaofGenesandGenomes(KEGG)analysis.Withintheseannotatedtranscripts,wecollected278sequencesrelatedtoinsecticideresistance.GOandKEGGanalysisofdifferentexpressiongenesbetweenTSWV-infectedandnon-infectedWFTpopulationrevealedthatTSWVcanregulatecellularprocessandimmuneresponse,whichmightleadtolowvirustitersinthripscellsandnodetrimentaleffectsonF.occidentalis.Thisdata-setnotonlyenrichesgenomicresourceforWFT,butalsobenefitsresearchintoitsmoleculargeneticsandfunctionalgenomics.

【 授权许可】

Unknown   

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