Activation of calcineurin is mainly responsible for the calcium sensitivity of gene deletion mutations in the genome of budding yeast

Genomics

Gang Zhao³  Jingcai Du³  Yunying Zhao²  Linghuo Jiang¹ 

[1] The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaThe National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, ChinaThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaDepartment of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China;The National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, ChinaThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

关键词: Calcium;    Magnesium;    Calcineurin;    VPS;    ESCRT;    Yeast;   

DOI  :  10.1016/j.ygeno.2012.09.005

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

Herewehaveidentified120genedeletionmutantsthataresensitiveto0.4#xA0;McalciuminSaccharomycescerevisiae.Twenty-sevenofthesemutantsareofgenesinvolvedinthevacuolarproteinsortingpathway,includingthoseencodingthesevencomponentsoftheESCRTcomplexes,andtenofthemencodethecomponentsandassemblyfactorsofthevacuolarH⁺-ATPase.BothMediatorandPaf1complexesmodulatingtheactivityofthegeneraltranscriptionmachineryareinvolvedinthecalciumsensitivityofyeastcells.Mostofthesemutantsshowelevatedintracellularcalciumcontentsinresponsetocalciumstress.Thecalciumsensitivityof106mutantscanbecompletelysuppressedby10#xA0;mMMg²⁺,56ofwhichcanalsobesuppressedbytheinhibitorofcalcineurin,cyclosporineA.Therefore,thecalciumsensitivityofnearlyahalfofthese120mutationsisatleastpartiallyduetotheactivationofcalcineurinandcanbemodulatedbymagnesiumion.

【 授权许可】

Unknown   

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