Laser ablation of the sonic hedgehog-a-expressing cells during fin regeneration affects ray branching morphogenesis

Developmental Biology

Uwe Strähle¹  Shirine Jeradi²  Jing Zhang²  Marie-Andrée Akimenko² 

[1] Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Postfach 3640, 76021 Karlsruhe, Germany;CAREG, Department of Biology, University of Ottawa, 30, Marie Curie, ON, Canada K1N 6N5

DOI  :  10.1016/j.ydbio.2012.03.008

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thezebrafishfinisanexcellentsystemtostudythemechanismsofdermalbonepatterning.Finraysaresegmentedstructuresthatformsuccessivebifurcationsbothduringontogenesisandregeneration.Previousstudiesshowedthatsonichedgehog(shha)mayregulateregenerativebonepatterningbasedonitsexpressionpatternandfunctionalanalysis.Thepresentstudyinvestigatestheroleoftheshha-expressingcellsinthepatterningoffinraybranches.Theshhaexpressiondomaininthebasalepidermisofeachfinraysplitsintotwopriortoraybifurcation.Inaddition,theosteoblastproliferationprofilefollowsthedynamicexpressionpatternofshha.Azebrafishtransgenicline,2.4shh:gfpABC#15,inwhichGFPexpressionrecapitulatestheendogenousexpressionofshha,wasusedtospecificallyablateshha-expressingcellswithalaserbeam.Suchablationsleadtoadelayinthesequenceofeventsleadingtoraybifurcationwithoutaffectingtheoverallgrowthofthefinray.Theseresultssuggestthatshha-expressingcellsdirectlocalizedosteoblastproliferationandthusregulatebranchingmorphogenesis.Thisstudyrevealsthefinrayasanewaccessiblesystemtoinvestigateepithelialndash;mesenchymalinteractionsleadingtoorganbranching.

【 授权许可】

Unknown   

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