Separate and distinctive roles for Wnt5a in tongue, lingual tissue and taste papilla development

Developmental Biology

Ken Iwatsuki¹  Hong-Xiang Liu²  Deborah L. Gumucio³  Ann S. Grosse³  Yuji Mishina²  Charlotte M. Mistretta² 

[1] Institute for Innovation, Ajinomoto Company, Kawasaki, Japan;Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109-1078, USA;Department of Cell and Developmental Biology, Medical School, University of Michigan, Ann Arbor MI 48109-2200, USA

DOI  :  10.1016/j.ydbio.2011.10.009

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

AlthoughcanonicalWntsignalingisknowntoregulatetastepapillainductionandnumbers,rolesfornoncanonicalWntpathwaysintongueandtastepapilladevelopmenthavenotbeenexplored.WithmutantmiceandwholetongueorgancultureswedemonstratethatWnt5aproteinandmessagearewithinanteriortonguemesenchymeacrossembryostagesfromtheinitiationoftongueformation,throughpapillaplacodeappearanceandtastepapilladevelopment.TheWnt5amutanttongueisseverelyshortened,withanankyloglossia,andlingualmesenchymeisdisorganized.However,fungiformpapillamorphology,numberandinnervationarepreserved,asisexpressionofthepapillamarker,Shh.Thesedatademonstratethatthegeneticregulationfortonguesizeandshapecanbeseparatedfromthatdirectinglingualpapilladevelopment.Preservednumberofpapillaeinashortenedtongueresultsinanincreaseddensityoffungiformpapillaeinthemutanttongues.Intongueorgancultures,exogenousWnt5aprofoundlysuppressespapillaformationandsimultaneouslydecreasescanonicalWntsignalingasmeasuredbytheTOPGALreporter.ThesefindingssuggestthatWnt5aantagonizescanonicalWntsignalingtodictatepapillanumberandspacing.Inall,distinctiverolesforWnt5aintonguesize,fungiformpapillapatterninganddevelopmentareshownandanecessarybalancebetweennon-canonicalandcanonicalWntpathsinregulatingtonguegrowthandfungiformpapillaeisproposedinamodel,throughtheRor2receptor.

【 授权许可】

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