Bmp4 in limb bud mesoderm regulates digit pattern by controlling AER development

Developmental Biology

Richard R. Behringer²  James F. Martin¹  Jennifer Selever¹  Mei-Fang Lu¹  Wei Liu¹ 

[1] Alkek Institute of Biosciences and Technology, Texas A&M System Health Science Center, Houston, TX 77030, United States;Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, United States

DOI  :  10.1016/j.ydbio.2004.08.024

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Inthedevelopinglimb,Bmp4isexpressedintheapicalectodermalridge(AER)andunderlyingmesoderm.InsightintothefunctionofBmp4inlimbdevelopmenthasbeenhamperedbytheearlyembryoniclethalityofBmp4nullembryos.WedirectlyinvestigatedBmp4usingaconditionalnullalleleofBmp4andthePrx1^cretransgenetoinactivateBmp4inlimbbudmesoderm.ThelimbbudmesodermofPrx1^cre;Bmp4mutantswasdefectiveinproductionofBmp4butstillcompetenttorespondtoBmpsignaling.Prx1^cre;Bmp4mutantembryoshaddefectivedigitpatterningincludinghindlimbpreaxialpolydactylywithposteriordigittransformations.ThePrx1^cre;Bmp4mutantsalsohadpostaxialpolydactylywithdigitfiveduplications.Bmp4mutantlimbshaddelayedinductionandmaturationoftheAERthatresultedinexpandedShhsignaling.Moreover,theAERpersistedlongerintheBmp4mutantlimbbudsexposingtheformingdigitstoprolongedFgf8signaling.OurdatashowthatBmp4inlimbmesodermregulatesAERinductionandmaturationandimplicatesignalingfromtheAERinregulationofdigitnumberandidentity.

【 授权许可】

Unknown   

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