Inhibition of myoblast migration via decorin expression is critical for normal skeletal muscle differentiation

Developmental Biology

Hugo C Olguin¹  Cristian Santander¹  Enrique Brandan¹ 

DOI  :  10.1016/S0012-1606(03)00180-5

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Duringlimbskeletalmuscleformation,committedmusclecellsproliferateanddifferentiateinthepresenceofextracellularsignalsthatstimulateorrepresseachprocess.Proteoglycansareextracellularmatrixorganizersandmodulatorsofgrowthfactoractivities,regulatingmuscledifferentiationinvitro.Previously,wecharacterizedproteoglycanexpressionduringearlylimbmuscleformationandshowedaspatiotemporalrelationbetweentheonsetofmyogenesisandtheexpressionofdecorin,animportantmuscleextracellularmatrixcomponentandpotentregulatorofTGF-βactivity.Toevaluatedecorinrsquo;sroleduringinvivodifferentiationincommittedmusclecells,wegraftedwildtypeanddecorin-nullmyoblastsontochicklimbbuds.Theabsenceofdecorinenhancedthemigrationanddistributionofmyoblastsinthelimb,correlatingwiththeinhibitionofskeletalmuscledifferentiation.Bothphenotypeswererevertedbydenovodecorinexpression.Invitro,wedeterminedthatbothdecorincoreproteinanditsglycosaminoglycanchainwererequiredtoreversethemigrationphenotype.Resultspresentedheresuggestthattheenhancedmigrationobservedindecorin-nullmyoblastsmaynotbedependentonchemotacticgrowthfactorsignalingnorthedifferentiationstatusofthecells.Decorinmaybeinvolvedintheestablishmentand/orcoordinationofacriticalmyoblastdensity,throughinhibitionofmigration,thatpermitsnormalmuscledifferentiationduringembryonicmyogenesis.

【 授权许可】

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