Tissue interactions pattern the mesenchyme of the embryonic mouse lung

Developmental Biology

Molly Weaver¹  Brigid L.M Hogan¹  Lorene Batts¹ 

DOI  :  10.1016/S0012-1606(03)00117-9

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Themechanismsthatcontrolproliferationanddifferentiationofembryoniclungmesenchymearelargelyunknown.WedescribeanexplantsysteminwhichexogenousrecombinantN-SonicHedgehog(N-Shh)proteinsustainsthesurvivalandproliferationoflungmesenchymeinadose-dependentmanner.Inaddition,Shhupregulatesseveralmesenchymalcellmarkers,includingitstargetgenePatched(Ptc),intercellularsignalinggenesBoneMorphogeneticProtein-4(Bmp4)andNoggin(Nog),andsmoothmuscleactinandmyosin.InexplantsexposedtoN-Shhinthemedium,theseproductsareupregulatedthroughoutthemesenchyme,butnotintheperiphery.Thisexclusionzonecorrelateswiththepresenceofanoverlyingmesotheliallayer,which,asinvivo,expressesFibroblastGrowthFactor9(Fgf9).RecombinantFgf9proteininhibitsthedifferentiationresponseofthemesenchymetoN-Shh,butdoesnotaffectproliferation.Weproposeamodelforhowfactorsmadebytwoepithelialcellpopulations,theinnerendodermandtheouterjacketofmesothelium,coordinatelyregulatetheproliferationanddifferentiationofthelungmesoderm.

【 授权许可】

Unknown   

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