Neuronal Differentiation from Postmitotic Precursors in the Ciliary Ganglion

Developmental Biology

Vivian M. Lee¹  John W. Sechrist¹  Rae Nishi¹  Marianne Bronner-Fraser¹ 

[1] Division of Biology, 139-74, California Institute of Technology, Pasadena, California, 91125

关键词: ciliary ganglion;    parasympathetic;    neuronal differentiation;    quail;    Islet-1;    HuD;    transplantation;    neurogenesis;    neural crest;   

DOI  :  10.1006/dbio.2002.0882

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Inthechickciliaryganglion,neuronalnumberiskeptconstantbetweenSt.29andSt.34(E6–E8)despitealargeamountofcelldeath.Here,wecharacterizethesourceofneurogeniccellsintheganglionasundifferentiatedneuralcrest-derivedcells.AtSt.29,neuronsandnonneuronalcellsintheciliaryganglionexpressedtheneuralcrestmarkersHNK-1andp75NTR.Over50%ofthecellswereneuronsatSt.29;ofthenonneuronalcells,asmallpopulationexpressedglialmarkers,whereasthemajoritywasundifferentiated.Whenplacedinculture,nonneuronalcellsacquiredimmunoreactivityforHuD,suggestingthattheyhadcommencedneuronaldifferentiation.Thenewlydifferentiatedneuronsarosefromprecursorsthatdidnotincorporatebromodeoxyuridine.Totestwhethertheseprecursorscouldundergoneuraldifferentiationinvivo,purifiednonneuronalcellsfromSt.29quailgangliaweretransplantedintochickembryosatSt.9–14.Subsequently,quailcellsexpressingneuronalmarkerswerefoundinthechickciliaryganglion.TheexistenceofthisprecursorpoolwastransientbecausenonneuronalcellsisolatedfromSt.38gangliafailedtoformneurons.SinceallciliaryganglionneuronsarebornpriortoSt.29,theseresultsdemonstratethattherearepostmitoticneuralcrest-derivedprecursorsinthedevelopingciliaryganglionthatcandifferentiateintoneuronsintheappropriateenvironment.

【 授权许可】

Unknown