Discrimination of the Roles of MPF and MAP Kinase in Morphological Changes That Occur during Oocyte Maturation

Developmental Biology

Masakane Yamashita¹  Tomoya Kotani¹ 

[1] Laboratory of Molecular and Cellular Interactions, Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Japan

关键词: cyclin B;    microtubules;    chromosome condensation;    spindle formation;    Rana oocytes;   

DOI  :  10.1006/dbio.2002.0853

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Maturingamphibianoocytesundergodrasticmorphologicalchanges,includinggerminalvesiclebreakdown(GVBD),chromosomecondensation,andspindleformationinresponsetoprogesterone.Twokinases,maturation-promotingfactor(MPF)andmitogen-activatedproteinkinase(MAPK),areinvolvedinthesechanges,buttheirpreciserolesareunknown.UnlikeinXenopusoocytes,discriminationofthefunctionsofMAPKandMPFinRanaoocytesiseasyowingtothelackofpre-MPF.WeinvestigatedtherolesofthesekinasesbycarefulobservationsofchromosomesandmicrotubulesinRanaoocytes.MPFandMAPKactivitiesweremanipulatedbytreatmentwithprogesterone,c-mosmRNA,orcyclinBmRNAincombinationwithMAPKkinaseinhibitors.Activationofonekinasewithoutactivationoftheotherinducedonlylimitedevents;GVBDwasinducedbyMPFwithoutMAPK,andreorganizationofmicrotubulesatGVBDwasinducedbyMAPKwithoutMPF,butothereventswerenotinduced.Incontrast,coactivationofMPFandMAPKbyinjectionofc-mosandcyclinBmRNApromotedalmostallofthemorphologicalchangesthatoccurduringmaturationwithoutprogesterone,indicatingthatthesearecontrolledbycooperationofMPFandMAPK.TheresultsrevealedthefunctionsofMAPKandMPFineachprocessofsequentialmorphologicalchangesduringoocytematuration.

【 授权许可】

Unknown