A Novel Invertebrate Trophic Factor Related to Invertebrate Neurotrophins Is Involved in Planarian Body Regional Survival and Asexual Reproduction

Developmental Biology

Juana Fernàndez-Rodríguez¹  David Bueno¹  Rafael Romero¹  Victor Hernàndez-Hernàndez¹  Albert Cardona¹ 

[1] Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, E-08028, Barcelona, Catalonia, Spain

关键词: planarian;    trophic factor;    regeneration;    asexual reproduction;    neurotrophin;   

DOI  :  10.1006/dbio.2002.0851

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Trophicfactorsareaheterogeneousgroupofmoleculesthatpromotecellgrowthandsurvival.Infreshwaterplanarians,thesmallsecretedproteinTCEN49islinkedtotheregionalmaintenanceoftheplanariancentralbodyregion.Toinvestigateitsfunctioninvivo,weperformedloss-of-functionandgain-of-functionexperimentsbyRNAinterferenceandbytheimplantationofmicrobeadssoakedinTCEN49,respectively.WeshowthatTCEN49behavesasatrophicfactorinvolvedincentralbodyregionneuronsurvival.Inplanariantailregenerates,tcen49expressioninhibitionbydouble-strandedRNAinterferencecausesextensiveapoptosisinvariouscelltypes,includingnervecells.ThisphenotypeisrescuedbytheimplantationofmicrobeadssoakedinTCEN49afterRNAinterference.Ontheotherhand,inorganismscommittedtoasexualreproduction,bothtcen49mRNAanditsproteinaredetectednotonlyinthecentralbodyregionbutalsointheposteriorregion,expandingfromcellsclosetotheventralnervechords.Insomecases,theimplantationofmicrobeadssoakedinTCEN49intheposteriorbodyregiondrivesorganismstoreproduceasexually,andtheinhibitionoftcen49expressionobstructsthisprocess,suggestingalinkbetweenthecentralnervoussystem,TCEN49,regionalinduction,andasexualreproduction.Finally,thedistributionofTCEN49cysteineandtyrosineresiduesalsopointstoacommonevolutionaryoriginforTCEN49andmolluscanneurotrophins.

【 授权许可】

Unknown